Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 148282..149069 Replicon plasmid pRetKim5b
Accession NZ_CP021126
Organism Rhizobium sp. Kim5

Toxin (Protein)


Gene name TacT2 Uniprot ID -
Locus tag KIM5_RS25525 Protein ID WP_077989752.1
Coordinates 148575..149069 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID -
Locus tag KIM5_RS25520 Protein ID WP_077989753.1
Coordinates 148282..148578 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM5_RS25500 143755..144528 - 774 WP_086156558.1 hypothetical protein -
KIM5_RS34405 145295..145750 - 456 WP_164519060.1 hypothetical protein -
KIM5_RS25515 146058..148175 + 2118 WP_086156559.1 carboxy terminal-processing peptidase -
KIM5_RS25520 148282..148578 + 297 WP_077989753.1 DUF1778 domain-containing protein Antitoxin
KIM5_RS25525 148575..149069 + 495 WP_077989752.1 GNAT family N-acetyltransferase Toxin
KIM5_RS25530 149199..150095 + 897 WP_009986653.1 GGDEF domain-containing protein -
KIM5_RS25535 150118..150486 - 369 WP_077989751.1 hypothetical protein -
KIM5_RS25540 150516..150989 - 474 WP_077989750.1 hypothetical protein -
KIM5_RS25545 151009..152229 - 1221 WP_077989749.1 hypothetical protein -
KIM5_RS25550 152272..153036 - 765 WP_077989748.1 TfuA-like protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..482471 482471


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(32-142)

Antitoxin

Vibrio phage ICP1, Orf50

(16-89)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 17714.29 Da        Isoelectric Point: 9.8090

>T76019 WP_077989752.1 NZ_CP021126:148575-149069 [Rhizobium sp. Kim5]
VTFSPPAPLADHHELAEFNSGVDELDEWLRRRARANQAGGASRTFVVCRGNRVIAYYALASGAVRQPEAPGRFRRNMPDP
VPVAVLGRLAIDQSHQGRGIGRALVRDAGLRLLNAAEILGIRGLLVHAISDGARAFYEAVGFLPSPSDPMMLMVGLHDLN
SALR

Download         Length: 495 bp

>T76019 NZ_CP021126:148575-149069 [Rhizobium sp. Kim5]
GTGACTTTCAGCCCGCCGGCGCCGTTGGCCGATCATCACGAGCTGGCCGAATTCAATTCCGGCGTGGATGAACTGGATGA
GTGGCTGCGTCGCCGCGCCCGCGCCAATCAGGCAGGCGGCGCGTCGCGCACCTTCGTCGTCTGCCGGGGAAACCGGGTCA
TCGCCTATTACGCGCTCGCCTCCGGGGCGGTGCGGCAGCCGGAAGCGCCTGGCCGTTTTCGGCGGAACATGCCCGATCCG
GTTCCGGTTGCCGTGCTCGGCCGCCTCGCCATCGATCAATCCCACCAGGGGCGCGGTATCGGCAGGGCATTGGTGCGCGA
TGCGGGCCTGCGCCTGCTCAACGCCGCAGAAATCCTCGGAATCCGCGGTCTGCTGGTGCATGCGATTTCCGACGGCGCGC
GCGCCTTCTATGAGGCGGTCGGCTTCCTGCCCTCGCCATCCGATCCCATGATGCTGATGGTCGGGTTGCATGATCTCAAC
AGCGCGCTGCGCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11059.59 Da        Isoelectric Point: 5.2300

>AT76019 WP_077989753.1 NZ_CP021126:148282-148578 [Rhizobium sp. Kim5]
VPASSTNKNSEASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEIYAEYLARLDAPAE
PNERLKRTMLTKAPWDEA

Download         Length: 297 bp

>AT76019 NZ_CP021126:148282-148578 [Rhizobium sp. Kim5]
GTGCCCGCCTCGTCTACAAACAAAAATTCAGAGGCAAGCGTGCCGCTGAATATGCGCATCAAGCCCGCGACCCGCAATTT
GATCGACCGCGCCGCCGAGCTGCTTGGCAAGACACGCACGGATTTCATGCTGGAGGCTTCGGAGCGGCGCGCCGAGGAGG
TGTTGCTCGACCGGACCGTATTCACGGTCAGCCCCGAGATCTATGCCGAATATCTTGCCCGCCTTGATGCGCCGGCTGAG
CCCAATGAGCGTCTGAAACGGACGATGTTGACCAAAGCGCCATGGGATGAAGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References