Detailed information of TA system TA076019

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 148282..149069
• Replicon: plasmid pRetKim5b
• Accession: NZ_CP021126
• Organism: Rhizobium sp. Kim5

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: -
• Locus tag: KIM5_RS25525
• Protein ID: WP_077989752.1
• Coordinates: 148575..149069 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: -
• Locus tag: KIM5_RS25520
• Protein ID: WP_077989753.1
• Coordinates: 148282..148578 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM5_RS25500	143755..144528	-	774	WP_086156558.1	hypothetical protein	-
KIM5_RS34405	145295..145750	-	456	WP_164519060.1	hypothetical protein	-
KIM5_RS25515	146058..148175	+	2118	WP_086156559.1	carboxy terminal-processing peptidase	-
KIM5_RS25520	148282..148578	+	297	WP_077989753.1	DUF1778 domain-containing protein	Antitoxin
KIM5_RS25525	148575..149069	+	495	WP_077989752.1	GNAT family N-acetyltransferase	Toxin
KIM5_RS25530	149199..150095	+	897	WP_009986653.1	GGDEF domain-containing protein	-
KIM5_RS25535	150118..150486	-	369	WP_077989751.1	hypothetical protein	-
KIM5_RS25540	150516..150989	-	474	WP_077989750.1	hypothetical protein	-
KIM5_RS25545	151009..152229	-	1221	WP_077989749.1	hypothetical protein	-
KIM5_RS25550	152272..153036	-	765	WP_077989748.1	TfuA-like protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..482471	482471

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (32-142)

Antitoxin

• Vibrio phage ICP1, Orf50 (16-89)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 17714.29 Da
• Isoelectric Point: 9.8090

>T76019 WP_077989752.1 NZ_CP021126:148575-149069 [Rhizobium sp. Kim5]
VTFSPPAPLADHHELAEFNSGVDELDEWLRRRARANQAGGASRTFVVCRGNRVIAYYALASGAVRQPEAPGRFRRNMPDP
VPVAVLGRLAIDQSHQGRGIGRALVRDAGLRLLNAAEILGIRGLLVHAISDGARAFYEAVGFLPSPSDPMMLMVGLHDLN
SALR

Nucleotide

• Length: 495 bp

>T76019 NZ_CP021126:148575-149069 [Rhizobium sp. Kim5]
GTGACTTTCAGCCCGCCGGCGCCGTTGGCCGATCATCACGAGCTGGCCGAATTCAATTCCGGCGTGGATGAACTGGATGA
GTGGCTGCGTCGCCGCGCCCGCGCCAATCAGGCAGGCGGCGCGTCGCGCACCTTCGTCGTCTGCCGGGGAAACCGGGTCA
TCGCCTATTACGCGCTCGCCTCCGGGGCGGTGCGGCAGCCGGAAGCGCCTGGCCGTTTTCGGCGGAACATGCCCGATCCG
GTTCCGGTTGCCGTGCTCGGCCGCCTCGCCATCGATCAATCCCACCAGGGGCGCGGTATCGGCAGGGCATTGGTGCGCGA
TGCGGGCCTGCGCCTGCTCAACGCCGCAGAAATCCTCGGAATCCGCGGTCTGCTGGTGCATGCGATTTCCGACGGCGCGC
GCGCCTTCTATGAGGCGGTCGGCTTCCTGCCCTCGCCATCCGATCCCATGATGCTGATGGTCGGGTTGCATGATCTCAAC
AGCGCGCTGCGCTGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11059.59 Da
• Isoelectric Point: 5.2300

>AT76019 WP_077989753.1 NZ_CP021126:148282-148578 [Rhizobium sp. Kim5]
VPASSTNKNSEASVPLNMRIKPATRNLIDRAAELLGKTRTDFMLEASERRAEEVLLDRTVFTVSPEIYAEYLARLDAPAE
PNERLKRTMLTKAPWDEA

Nucleotide

• Length: 297 bp

>AT76019 NZ_CP021126:148282-148578 [Rhizobium sp. Kim5]
GTGCCCGCCTCGTCTACAAACAAAAATTCAGAGGCAAGCGTGCCGCTGAATATGCGCATCAAGCCCGCGACCCGCAATTT
GATCGACCGCGCCGCCGAGCTGCTTGGCAAGACACGCACGGATTTCATGCTGGAGGCTTCGGAGCGGCGCGCCGAGGAGG
TGTTGCTCGACCGGACCGTATTCACGGTCAGCCCCGAGATCTATGCCGAATATCTTGCCCGCCTTGATGCGCCGGCTGAG
CCCAATGAGCGTCTGAAACGGACGATGTTGACCAAAGCGCCATGGGATGAAGCGTGA