Detailed information of TA system TA076017

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3034086..3034759
• Replicon: chromosome
• Accession: NZ_CP021124
• Organism: Rhizobium sp. Kim5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: KIM5_RS15250
• Protein ID: WP_077993003.1
• Coordinates: 3034086..3034499 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A060I987
• Locus tag: KIM5_RS15255
• Protein ID: WP_010061195.1
• Coordinates: 3034496..3034759 (-)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KIM5_RS15240	3031578..3032777	-	1200	WP_077992994.1	efflux RND transporter periplasmic adaptor subunit	-
KIM5_RS15245	3033052..3033963	-	912	WP_077993001.1	adenylate/guanylate cyclase domain-containing protein	-
KIM5_RS15250	3034086..3034499	-	414	WP_077993003.1	type II toxin-antitoxin system VapC family toxin	Toxin
KIM5_RS15255	3034496..3034759	-	264	WP_010061195.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
KIM5_RS15260	3034838..3036388	-	1551	WP_039620973.1	SpoVR family protein	-
KIM5_RS15265	3036385..3037659	-	1275	WP_038690084.1	YeaH/YhbH family protein	-
KIM5_RS15270	3037672..3039615	-	1944	WP_038690085.1	PrkA family serine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-57)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15142.57 Da
• Isoelectric Point: 6.8720

>T76017 WP_077993003.1 NZ_CP021124:c3034499-3034086 [Rhizobium sp. Kim5]
MTAWMLDTNIASHVIKGDRPEILKRLATLPMDEIVISSITEGELLYGLAKRGYPKALSERVRQFLLRVDVLPWDHDVTRT
YGDLRAACEAKGTILAPLDMMIAAHAVATAATLVTRDKAFAHISEPLKVDDWTGGRS

Nucleotide

• Length: 414 bp

>T76017 NZ_CP021124:c3034499-3034086 [Rhizobium sp. Kim5]
ATGACGGCATGGATGCTCGACACCAATATCGCCAGTCATGTCATCAAAGGCGATCGGCCGGAGATACTTAAAAGACTGGC
GACGCTTCCAATGGACGAGATCGTCATCTCATCGATCACGGAAGGGGAATTGCTCTACGGTCTTGCCAAGCGAGGCTATC
CGAAGGCGCTATCCGAGCGGGTAAGGCAATTCCTTCTCAGGGTCGATGTTCTCCCCTGGGATCACGACGTCACGCGAACC
TATGGTGACCTGCGCGCCGCCTGTGAAGCCAAAGGCACGATTCTGGCGCCCCTGGACATGATGATTGCCGCTCATGCCGT
CGCGACCGCGGCAACCCTCGTCACGCGTGACAAGGCTTTCGCTCACATTTCTGAGCCTTTAAAGGTCGATGACTGGACGG
GCGGAAGAAGCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10119.33 Da
• Isoelectric Point: 5.8867

>AT76017 WP_010061195.1 NZ_CP021124:c3034759-3034496 [Rhizobium sp. Kim5]
MATKTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKTGDVILSRKPDSWDGLFELHRKDPIPDDFLGPADRRQPSQDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT76017 NZ_CP021124:c3034759-3034496 [Rhizobium sp. Kim5]
ATGGCAACGAAAACGGCTAAGATTTTTACGACCGGCCGCAGCCAGGCGGTGAGGCTCCCTGCGGAGTTTCGCTTTGAGGA
GAGCGAAGTGTTCGTTCGTCGTGATCCGAAGACCGGTGATGTCATATTATCGCGCAAGCCGGATTCATGGGATGGCCTTT
TCGAACTCCATCGGAAGGATCCGATTCCGGACGATTTCCTTGGACCGGCCGATCGTCGCCAACCTTCGCAAGATCGGGAT
CCGTTCGAAGGTTGGAAGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A060I987