Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 360960..361597 Replicon chromosome
Accession NZ_CP021124
Organism Rhizobium sp. Kim5

Toxin (Protein)


Gene name vapC Uniprot ID A0A060HRP4
Locus tag KIM5_RS01780 Protein ID WP_010047998.1
Coordinates 360960..361358 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A060HVL6
Locus tag KIM5_RS01785 Protein ID WP_038686430.1
Coordinates 361355..361597 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KIM5_RS01755 356181..356993 + 813 WP_077991234.1 class II glutamine amidotransferase -
KIM5_RS01760 357024..358232 - 1209 WP_077991233.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
KIM5_RS01765 358332..359213 - 882 WP_009992620.1 LysR family transcriptional regulator -
KIM5_RS01770 359305..360555 + 1251 WP_077991231.1 MFS transporter -
KIM5_RS01775 360552..360938 + 387 WP_009988872.1 VOC family protein -
KIM5_RS01780 360960..361358 - 399 WP_010047998.1 type II toxin-antitoxin system VapC family toxin Toxin
KIM5_RS01785 361355..361597 - 243 WP_038686430.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
KIM5_RS01790 361651..363312 - 1662 WP_077991230.1 urocanate hydratase -
KIM5_RS01795 363549..363878 - 330 WP_009994542.1 TfoX/Sxy family protein -
KIM5_RS01800 363882..364979 - 1098 WP_077991229.1 DUF917 domain-containing protein -
KIM5_RS01805 364960..366240 - 1281 WP_077991228.1 Zn-dependent hydrolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-122)

Antitoxin

SpoVT-AbrB domain

(7-44)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14686.83 Da        Isoelectric Point: 6.2354

>T76015 WP_010047998.1 NZ_CP021124:c361358-360960 [Rhizobium sp. Kim5]
MKYLLDTNAVIALIKGNDRLIAELRKHTPTDFAMPAIVAHELFYGAYKSLHIDENLARIDALQFAILEFDRNDARRAGEV
RAALRASGTPIGPYDVLIAGQAAARGLVLITRNLREFERVADLQVENWEGSP

Download         Length: 399 bp

>T76015 NZ_CP021124:c361358-360960 [Rhizobium sp. Kim5]
ATGAAGTACCTGCTCGATACTAATGCCGTCATTGCCTTGATAAAGGGCAATGACCGCCTCATCGCCGAACTGCGCAAGCA
CACACCAACGGATTTCGCCATGCCGGCGATCGTCGCGCATGAACTCTTTTACGGCGCTTACAAAAGTTTGCATATCGATG
AGAATCTGGCGCGGATCGATGCGCTACAATTCGCCATACTGGAATTTGACCGGAACGACGCGCGCAGGGCCGGAGAAGTT
CGCGCAGCGCTGCGAGCATCGGGAACGCCCATCGGCCCCTATGACGTCCTGATTGCCGGGCAGGCCGCTGCGCGCGGCCT
CGTCCTGATCACCCGCAATCTGAGGGAGTTCGAGCGCGTGGCGGACCTGCAGGTAGAGAATTGGGAAGGCTCGCCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9179.25 Da        Isoelectric Point: 4.3063

>AT76015 WP_038686430.1 NZ_CP021124:c361597-361355 [Rhizobium sp. Kim5]
METAKIFWSGRSQAVRLPKEFRFDGDSVTIRRQGRAIVLEPISDNWDWLADVIGPVDPDFATAAEEQPAQQERPDLDFFK

Download         Length: 243 bp

>AT76015 NZ_CP021124:c361597-361355 [Rhizobium sp. Kim5]
ATGGAAACCGCAAAAATCTTCTGGTCAGGGCGTTCGCAAGCCGTCCGCTTGCCGAAGGAATTCCGCTTTGATGGCGATAG
TGTCACCATCCGGCGCCAAGGGCGTGCGATCGTTCTCGAACCGATTTCCGACAATTGGGATTGGCTGGCTGACGTGATCG
GTCCGGTCGATCCCGACTTCGCGACAGCAGCAGAAGAGCAGCCTGCGCAACAGGAACGACCTGACCTCGACTTCTTCAAA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060HRP4

Antitoxin

Source ID Structure
AlphaFold DB A0A060HVL6

References