Detailed information of TA system TA076002

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PP_4151-4152/excise(antitoxin)
• Location: 4118266..4119308
• Replicon: chromosome
• Accession: NZ_CP021111
• Organism: Bordetella genomosp. 13 strain AU7206

Toxin (Protein)

• Gene name: PP_4152
• Uniprot ID: -
• Locus tag: CAL15_RS18515
• Protein ID: WP_003109777.1
• Coordinates: 4118733..4119308 (+)
• Length: 192 a.a.

Antitoxin (Protein)

• Gene name: PP_4151
• Uniprot ID: I3TV68
• Locus tag: CAL15_RS18510
• Protein ID: WP_003050245.1
• Coordinates: 4118266..4118736 (+)
• Length: 157 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAL15_RS18475	4113658..4115076	-	1419	WP_020924934.1	TIGR03752 family integrating conjugative element protein	-
CAL15_RS18480	4115066..4115977	-	912	WP_003105643.1	TIGR03749 family integrating conjugative element protein	-
CAL15_RS18485	4115974..4116666	-	693	WP_003105641.1	TIGR03746 family integrating conjugative element protein	-
CAL15_RS18490	4116663..4117061	-	399	WP_003105639.1	TIGR03750 family conjugal transfer protein	-
CAL15_RS18495	4117073..4117432	-	360	WP_003090173.1	TIGR03745 family integrating conjugative element membrane protein	-
CAL15_RS18500	4117449..4117682	-	234	WP_003050225.1	TIGR03758 family integrating conjugative element protein	-
CAL15_RS18505	4117679..4118062	-	384	WP_003120001.1	RAQPRD family integrative conjugative element protein	-
CAL15_RS18510	4118266..4118736	+	471	WP_003050245.1	helix-turn-helix domain-containing protein	Antitoxin
CAL15_RS18515	4118733..4119308	+	576	WP_003109777.1	PIN domain-containing protein	Toxin
CAL15_RS18520	4119326..4120240	+	915	WP_003105629.1	AAA family ATPase	-
CAL15_RS18525	4120237..4120707	+	471	WP_003105626.1	hypothetical protein	-
CAL15_RS18530	4120704..4121204	+	501	WP_003090159.1	hypothetical protein	-
CAL15_RS18535	4121204..4122106	+	903	WP_003105624.1	nucleotidyltransferase domain-containing protein	-
CAL15_RS18540	4122145..4122870	+	726	WP_003050273.1	CBASS effector endonuclease NucC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	4068886..4168838	99952

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (9-114)

Antitoxin

• Helix-turn-helix domain, group 17 (85-133)

Sequences

Toxin

Protein

• Length: 192 a.a.
• Molecular weight: 21644.79 Da
• Isoelectric Point: 5.6172

>T76002 WP_003109777.1 NZ_CP021111:4118733-4119308 [Bordetella genomosp. 13]
MRHSPFTAVYDACVLYPAPLRDFLMWLGLSGRFRARWSQAIHEEWKRNLLINRPDLTRVQVDRTSDLMDRAIPDGLVEGY
EALVAGLTLPDPDDRHVLAAAIRCGASVIVTFNERDFPNDLLAPYGIESQHPDEFVDNLLDLDAAAVVSAAQRQRAQLKH
PPIEVDRYLEILLRQGLVQTTKVLATYRTIL

Nucleotide

• Length: 576 bp

>T76002 NZ_CP021111:4118733-4119308 [Bordetella genomosp. 13]
ATGAGGCATTCCCCATTCACCGCCGTCTACGACGCGTGCGTTCTATATCCCGCGCCACTGCGGGATTTCCTGATGTGGCT
CGGCCTGTCTGGCCGCTTCCGGGCGCGGTGGAGCCAAGCCATTCATGAGGAGTGGAAACGCAACCTGTTGATCAACCGCC
CCGATCTCACCCGGGTTCAGGTCGACAGGACGTCGGATCTCATGGACCGGGCAATTCCGGACGGCTTGGTGGAGGGCTAC
GAAGCGCTCGTGGCAGGCCTGACATTGCCCGATCCGGACGACCGGCACGTCCTGGCTGCGGCGATTCGCTGCGGTGCGAG
CGTGATTGTGACGTTCAACGAACGGGATTTCCCGAACGATCTGCTGGCTCCGTACGGCATCGAATCGCAGCACCCCGATG
AGTTTGTGGACAACCTACTGGATCTGGACGCGGCCGCCGTGGTGTCGGCTGCGCAGCGCCAGCGTGCCCAACTCAAGCAT
CCGCCGATCGAGGTGGACCGCTATCTCGAAATCCTGCTGCGCCAGGGCCTTGTGCAAACGACCAAGGTGCTGGCGACCTA
TCGCACCATTCTCTGA

Antitoxin

Protein

• Length: 157 a.a.
• Molecular weight: 17245.62 Da
• Isoelectric Point: 6.3803

>AT76002 WP_003050245.1 NZ_CP021111:4118266-4118736 [Bordetella genomosp. 13]
MTTATAQSKMTLPAAGEVKAAVQGQRALAAYLATQFETQHIQIFDDHKQAHQVELPTSALRLLVDILAELADGNAVKVVP
VHAELTTQEAADLLNVSRPHFVKLLEDGVLAFHRTGKHRRVRFADLMQYKEARERASEQAMAELAQQSQELGMGYE

Nucleotide

• Length: 471 bp

>AT76002 NZ_CP021111:4118266-4118736 [Bordetella genomosp. 13]
ATGACCACCGCCACTGCCCAATCCAAGATGACCCTTCCCGCCGCGGGAGAGGTGAAGGCAGCCGTCCAGGGTCAACGTGC
CTTGGCGGCTTACCTCGCAACCCAGTTCGAGACGCAGCACATCCAGATCTTTGATGACCACAAACAGGCTCATCAGGTGG
AACTGCCTACCTCGGCTTTACGCCTGCTGGTCGACATCCTGGCCGAGTTGGCCGATGGCAATGCAGTAAAGGTGGTGCCC
GTCCATGCAGAGCTGACGACCCAAGAGGCGGCGGACTTGCTCAACGTGTCCCGTCCCCATTTCGTCAAGCTGCTCGAAGA
TGGGGTGTTGGCATTTCATCGCACCGGCAAGCACCGCAGGGTGAGGTTCGCCGATCTGATGCAGTACAAGGAAGCGCGCG
AGCGCGCCAGTGAGCAGGCGATGGCCGAACTCGCTCAGCAGTCGCAAGAGTTGGGAATGGGATACGAATGA

Structures

Antitoxin

• AlphaFold DB: I3TV68