Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 99276..99856 Replicon chromosome
Accession NZ_CP021106
Organism Nitrosospira lacus strain APG3

Toxin (Protein)


Gene name mazF Uniprot ID A0A1W6SKP1
Locus tag EBAPG3_RS00435 Protein ID WP_040853119.1
Coordinates 99518..99856 (+) Length 113 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A1W6SKP5
Locus tag EBAPG3_RS00430 Protein ID WP_004180535.1
Coordinates 99276..99518 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EBAPG3_RS00405 94477..95574 - 1098 WP_040853117.1 glycosyltransferase -
EBAPG3_RS00410 95649..96470 + 822 WP_004180530.1 phytanoyl-CoA dioxygenase family protein -
EBAPG3_RS00415 96532..97197 + 666 WP_051049106.1 WbqC family protein -
EBAPG3_RS00420 97273..97989 - 717 WP_004180532.1 acetyltransferase -
EBAPG3_RS00425 98113..99066 + 954 WP_004180534.1 glycosyltransferase family 2 protein -
EBAPG3_RS00430 99276..99518 + 243 WP_004180535.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
EBAPG3_RS00435 99518..99856 + 339 WP_040853119.1 endoribonuclease MazF Toxin
EBAPG3_RS00440 99951..100847 + 897 WP_151898973.1 glycosyltransferase family 2 protein -
EBAPG3_RS00445 101003..101893 + 891 WP_040853121.1 SDR family oxidoreductase -
EBAPG3_RS00450 102060..102479 + 420 WP_040853181.1 aminoacyl-tRNA hydrolase -
EBAPG3_RS00455 102539..104047 - 1509 WP_004180542.1 HAMP domain-containing histidine kinase -
EBAPG3_RS00460 104075..104548 - 474 WP_004180552.1 response regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(9-107)

Antitoxin

Antitoxin PemI-like

(1-79)


Sequences

Toxin        


Download         Length: 113 a.a.        Molecular weight: 12449.49 Da        Isoelectric Point: 9.9018

>T75985 WP_040853119.1 NZ_CP021106:99518-99856 [Nitrosospira lacus]
MVHRRVPEAGDIVWLHFDPQAGHEQAGHRPALVVSPSSYNGKTGLMLCCPITTRVKGYPFEVLLSEDRPSAVLADQIKSL
DWVARKARYKGKVSRSELSQVLAKISVLIEKL

Download         Length: 339 bp

>T75985 NZ_CP021106:99518-99856 [Nitrosospira lacus]
ATGGTTCATCGCCGTGTCCCTGAGGCTGGCGACATTGTGTGGCTACACTTCGACCCGCAAGCTGGACACGAGCAGGCTGG
GCACCGCCCGGCGCTCGTGGTTAGCCCATCGTCCTATAATGGCAAAACCGGCCTTATGTTGTGTTGCCCAATAACGACGC
GGGTTAAGGGGTACCCTTTCGAGGTTCTGCTTTCTGAAGATCGTCCCAGTGCTGTTCTAGCCGACCAAATTAAAAGTCTC
GATTGGGTTGCACGTAAAGCCCGCTACAAAGGCAAGGTCTCGCGCAGCGAATTATCCCAAGTGCTGGCAAAAATTTCCGT
TCTCATTGAAAAACTATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8772.16 Da        Isoelectric Point: 4.7723

>AT75985 WP_004180535.1 NZ_CP021106:99276-99518 [Nitrosospira lacus]
MRVIVKKWGNSASVRIPAGVMEAAHLSLDDPVDIREEEGRIIIEPICTHKYDLAQLLAGITPENLHGEVDFGVPVGKELL

Download         Length: 243 bp

>AT75985 NZ_CP021106:99276-99518 [Nitrosospira lacus]
ATGCGCGTAATCGTGAAGAAATGGGGAAATAGTGCATCCGTTCGCATCCCTGCCGGGGTCATGGAAGCTGCACATTTAAG
CCTGGATGACCCGGTTGACATTCGTGAAGAGGAAGGGCGCATCATCATCGAACCGATTTGCACACATAAATACGACCTCG
CTCAGTTGCTTGCCGGCATAACGCCAGAAAATCTGCATGGCGAAGTTGATTTCGGGGTGCCTGTCGGCAAAGAGCTTTTG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W6SKP1

Antitoxin

Source ID Structure
AlphaFold DB A0A1W6SKP5

References