Detailed information of TA system TA075977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2064986..2065515
• Replicon: chromosome
• Accession: NZ_CP021105
• Organism: Staphylococcus aureus strain CC5

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B4893_RS10655
• Protein ID: WP_000621175.1
• Coordinates: 2064986..2065348 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B4893_RS10660
• Protein ID: WP_000948331.1
• Coordinates: 2065345..2065515 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B4893_RS10630	2061964..2062734	-	771	WP_001041111.1	RNA polymerase sigma factor SigB	-
B4893_RS10635	2062709..2063188	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
B4893_RS10640	2063190..2063516	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B4893_RS10645	2063635..2064636	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B4893_RS10655	2064986..2065348	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B4893_RS10660	2065345..2065515	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B4893_RS10665	2065600..2066748	-	1149	WP_001281154.1	alanine racemase	-
B4893_RS10670	2066814..2067173	-	360	WP_000581197.1	holo-ACP synthase	-
B4893_RS10675	2067177..2067668	-	492	WP_001205912.1	PH domain-containing protein	-
B4893_RS10680	2067655..2069238	-	1584	WP_001294626.1	PH domain-containing protein	-
B4893_RS10685	2069231..2069710	-	480	WP_094659677.1	hypothetical protein	-
B4893_RS10690	2069918..2070478	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T75977 WP_000621175.1 NZ_CP021105:c2065348-2064986 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T75977 NZ_CP021105:c2065348-2064986 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT75977 WP_000948331.1 NZ_CP021105:c2065515-2065345 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT75977 NZ_CP021105:c2065515-2065345 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1