Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 55042..55564 Replicon plasmid p13P477T-7
Accession NZ_CP021103
Organism Escherichia coli strain 13P477T

Toxin (Protein)


Gene name relE Uniprot ID D0QMR1
Locus tag BWI89_RS28915 Protein ID WP_000220561.1
Coordinates 55042..55323 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag BWI89_RS28920 Protein ID WP_000121743.1
Coordinates 55313..55564 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI89_RS28885 50518..50754 - 237 Protein_60 hypothetical protein -
BWI89_RS28890 50775..51569 - 795 WP_000572405.1 aminoglycoside O-phosphotransferase APH(3')-IIa -
BWI89_RS28895 51598..53028 - 1431 Protein_62 IS4-like element IS50R family transposase -
BWI89_RS28900 53148..53336 + 189 WP_000957857.1 hypothetical protein -
BWI89_RS28905 53346..54545 + 1200 WP_000948429.1 IS91 family transposase -
BWI89_RS29550 54711..54803 + 93 Protein_65 IS6 family transposase -
BWI89_RS28915 55042..55323 - 282 WP_000220561.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BWI89_RS28920 55313..55564 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
BWI89_RS28930 56799..57638 + 840 WP_024129959.1 replication initiation protein -
BWI89_RS28935 57659..58501 + 843 WP_000435064.1 replication initiation protein -
BWI89_RS28940 58505..58951 + 447 WP_001074384.1 hypothetical protein -
BWI89_RS28945 59019..59441 + 423 WP_179292036.1 DNA distortion polypeptide 3 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid oqxA / oqxB / aph(3')-IIa - 1..80206 80206


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11023.86 Da        Isoelectric Point: 10.5938

>T75968 WP_000220561.1 NZ_CP021103:c55323-55042 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADMR

Download         Length: 282 bp

>T75968 NZ_CP021103:c55323-55042 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT75968 WP_000121743.1 NZ_CP021103:c55564-55313 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT75968 NZ_CP021103:c55564-55313 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S5I302

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References