Detailed information of TA system TA075956

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 141130..141707
• Replicon: plasmid unnamed2
• Accession: NZ_CP021077
• Organism: Burkholderia glumae strain 957856-41-c

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: B7759_RS30820
• Protein ID: WP_209954638.1
• Coordinates: 141342..141707 (+)
• Length: 122 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A158I2A1
• Locus tag: B7759_RS30815
• Protein ID: WP_060858320.1
• Coordinates: 141130..141348 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7759_RS30790 (B7759_06073)	136289..137155	-	867	WP_012732871.1	IS21-like element ISBcen28 family helper ATPase IstB	-
B7759_RS30795 (B7759_06074)	137152..138408	-	1257	WP_017423782.1	IS21 family transposase	-
B7759_RS30800 (B7759_06075)	138606..139082	+	477	WP_012734051.1	transposase	-
B7759_RS30805 (B7759_06076)	139058..139408	+	351	WP_012732753.1	IS66 family insertion sequence element accessory protein TnpB	-
B7759_RS30810 (B7759_06077)	139440..140996	+	1557	WP_012732752.1	IS66 family transposase	-
B7759_RS30815 (B7759_06078)	141130..141348	+	219	WP_060858320.1	ribbon-helix-helix protein, CopG family	Antitoxin
B7759_RS30820 (B7759_06079)	141342..141707	+	366	WP_209954638.1	type II toxin-antitoxin system VapC family toxin	Toxin
B7759_RS30825 (B7759_06080)	142013..144619	+	2607	WP_209954640.1	hypothetical protein	-
B7759_RS30830 (B7759_06081)	144715..145551	+	837	WP_209954642.1	hypothetical protein	-
B7759_RS30835 (B7759_06083)	145879..146706	-	828	Protein_133	IS66 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..168969	168969

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-106)

Antitoxin

• Ribbon-helix-helix protein, CopG (4-39)

Sequences

Toxin

Protein

• Length: 122 a.a.
• Molecular weight: 13400.54 Da
• Isoelectric Point: 7.0133

>T75956 WP_209954638.1 NZ_CP021077:141342-141707 [Burkholderia glumae]
MVKALFDTNILIDYLNGVGAAKKELARYEYRAISVITWMEVLVGASGEEDAAIRAWLDTFDVVAVDSAIANRAVEIRKAK
KIRLPDAIVWASAQVHSLLLVSRNTKDFPADEPGVRVPYKI

Nucleotide

• Length: 366 bp

>T75956 NZ_CP021077:141342-141707 [Burkholderia glumae]
ATGGTGAAGGCGCTCTTCGATACGAACATCCTCATTGACTATCTCAACGGGGTCGGCGCAGCCAAGAAAGAACTGGCGCG
CTATGAGTATCGCGCGATTAGCGTGATCACCTGGATGGAAGTGCTGGTCGGAGCATCCGGCGAGGAAGACGCCGCGATCC
GCGCATGGCTCGATACATTCGATGTCGTTGCAGTAGACAGTGCGATCGCCAATCGCGCCGTCGAGATTCGTAAGGCTAAA
AAGATTCGTCTGCCCGATGCCATCGTGTGGGCATCAGCACAGGTTCACTCGCTGCTGCTTGTTTCGAGAAATACGAAGGA
CTTTCCAGCAGACGAGCCGGGTGTCCGCGTGCCATACAAAATCTAG

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8259.39 Da
• Isoelectric Point: 5.0651

>AT75956 WP_060858320.1 NZ_CP021077:141130-141348 [Burkholderia glumae]
MSRILVDLPESQVDDLAVIVEAEQRPRAAVIRDAIEAYISQRKRALGTDVFGLWKSRKTDGLAYQQELRSEW

Nucleotide

• Length: 219 bp

>AT75956 NZ_CP021077:141130-141348 [Burkholderia glumae]
ATGAGTCGTATCCTGGTTGACCTGCCTGAGTCGCAGGTTGACGACCTCGCGGTGATTGTGGAGGCCGAACAGCGGCCCCG
CGCGGCCGTCATCCGCGACGCCATTGAGGCGTATATCTCGCAGCGCAAACGCGCGCTCGGAACCGACGTATTTGGGCTGT
GGAAGAGCCGGAAGACGGATGGCCTGGCGTACCAGCAGGAGCTTCGTTCCGAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A158I2A1