Detailed information of TA system TA075954

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 123944..124539
• Replicon: plasmid unnamed1
• Accession: NZ_CP021076
• Organism: Burkholderia glumae strain 957856-41-c

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: C5AN78
• Locus tag: B7759_RS29865
• Protein ID: WP_012734107.1
• Coordinates: 123944..124294 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: C5AN79
• Locus tag: B7759_RS29870
• Protein ID: WP_012734108.1
• Coordinates: 124288..124539 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7759_RS29840 (B7759_05875)	119166..119912	-	747	WP_232252306.1	energy transducer TonB	-
B7759_RS29845 (B7759_05876)	119944..122319	-	2376	WP_232252305.1	TonB-dependent receptor	-
B7759_RS29850 (B7759_05877)	122633..123097	-	465	WP_012734106.1	Lrp/AsnC family transcriptional regulator	-
B7759_RS29855	123320..123541	-	222	WP_017433768.1	hypothetical protein	-
B7759_RS29860 (B7759_05878)	123691..123885	-	195	WP_124837685.1	hypothetical protein	-
B7759_RS29865 (B7759_05879)	123944..124294	-	351	WP_012734107.1	type II toxin-antitoxin system ChpB family toxin	Toxin
B7759_RS29870 (B7759_05880)	124288..124539	-	252	WP_012734108.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B7759_RS29875 (B7759_05881)	124802..125209	-	408	WP_230674618.1	hypothetical protein	-
B7759_RS29880 (B7759_05882)	125312..127147	-	1836	WP_124837684.1	site-specific integrase	-
B7759_RS29885 (B7759_05883)	127150..127491	-	342	WP_012732710.1	hypothetical protein	-
B7759_RS29890 (B7759_05884)	127548..127793	-	246	WP_127913916.1	hypothetical protein	-
B7759_RS29895 (B7759_05885)	127790..128032	-	243	WP_124837682.1	hypothetical protein	-
B7759_RS29900 (B7759_05886)	128046..128330	-	285	WP_017432347.1	hypothetical protein	-
B7759_RS29905 (B7759_05887)	128737..129225	-	489	WP_012732893.1	GNAT family N-acetyltransferase	-
B7759_RS29910 (B7759_05888)	129222..129491	-	270	WP_012732894.1	DUF1778 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..181392	181392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• Antitoxin PemI-like (1-83)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12389.37 Da
• Isoelectric Point: 8.8909

>T75954 WP_012734107.1 NZ_CP021076:c124294-123944 [Burkholderia glumae]
MVKRVKFERGDIVRVSLSPTVGREQQGDFRPALVLSPAAFNALGVALVAPITQGGEFARFAGFAVPLSGSGTETQGVALV
NMVRMLDLEGRGARKIERAPVEVVEDALARLQTIIE

Nucleotide

• Length: 351 bp

>T75954 NZ_CP021076:c124294-123944 [Burkholderia glumae]
ATGGTGAAGCGGGTCAAGTTCGAGCGAGGCGATATCGTGCGAGTGAGTCTGAGTCCGACGGTCGGCCGTGAGCAGCAAGG
AGATTTTCGTCCCGCGCTTGTGCTCTCTCCGGCGGCGTTTAACGCGCTGGGTGTGGCGCTCGTTGCCCCGATTACCCAGG
GCGGTGAATTCGCCCGCTTTGCAGGTTTTGCGGTTCCGCTCTCCGGTTCGGGGACGGAGACACAGGGAGTGGCACTGGTG
AACATGGTACGCATGCTCGACTTGGAAGGACGCGGCGCACGCAAGATCGAACGCGCGCCCGTCGAAGTGGTAGAGGATGC
CCTGGCCCGTCTTCAAACGATTATTGAGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 8940.31 Da
• Isoelectric Point: 6.2420

>AT75954 WP_012734108.1 NZ_CP021076:c124539-124288 [Burkholderia glumae]
MELKIQKWGNSAAVRLPSVLLEQINASVGGSLNADVRPDGVLLTPARRKYALDDLVAQCDTKAPVPADMAAWGEIKPVGR
EAW

Nucleotide

• Length: 252 bp

>AT75954 NZ_CP021076:c124539-124288 [Burkholderia glumae]
ATGGAACTAAAGATTCAGAAATGGGGCAATAGCGCGGCGGTGCGTTTGCCGAGCGTATTGCTCGAACAGATCAACGCATC
GGTCGGCGGTTCGCTGAACGCGGACGTGCGGCCGGATGGGGTGCTACTGACCCCGGCCCGGCGGAAGTATGCGCTGGATG
ATCTCGTGGCTCAGTGCGACACGAAGGCGCCTGTTCCGGCCGACATGGCAGCCTGGGGCGAGATCAAGCCGGTGGGACGT
GAAGCATGGTGA

Structures

Toxin

• AlphaFold DB: A0A246MF70

Antitoxin

• AlphaFold DB: A0A246MFN5