Detailed information of TA system TA075952

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 119187..119701
• Replicon: plasmid pWSM1497
• Accession: NZ_CP021071
• Organism: Mesorhizobium sp. WSM1497

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: A9K65_RS32970
• Protein ID: WP_086081393.1
• Coordinates: 119187..119396 (+)
• Length: 70 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: A9K65_RS32975
• Protein ID: WP_064993496.1
• Coordinates: 119399..119701 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A9K65_RS32920	114247..114487	-	241	Protein_96	DUF982 domain-containing protein	-
A9K65_RS32935	115138..115362	-	225	WP_064993500.1	hypothetical protein	-
A9K65_RS32940	115562..116632	-	1071	WP_064993499.1	DUF2213 domain-containing protein	-
A9K65_RS32945	116850..117089	+	240	WP_027162342.1	hypothetical protein	-
A9K65_RS32950	117203..117457	+	255	WP_064993498.1	hypothetical protein	-
A9K65_RS32955	117578..118291	+	714	WP_064993508.1	Crp/Fnr family transcriptional regulator	-
A9K65_RS32960	118288..118527	-	240	WP_086081392.1	hypothetical protein	-
A9K65_RS32970	119187..119396	+	210	WP_086081393.1	addiction module killer protein	Toxin
A9K65_RS32975	119399..119701	+	303	WP_064993496.1	putative addiction module antidote protein	Antitoxin
A9K65_RS32980	119807..120298	+	492	WP_064993495.1	YidB family protein	-
A9K65_RS32985	120291..120749	+	459	WP_084015726.1	DUF4112 domain-containing protein	-
A9K65_RS32990	120838..121089	+	252	WP_064993493.1	GlsB/YeaQ/YmgE family stress response membrane protein	-
A9K65_RS35805	121355..121516	-	162	WP_198327721.1	hypothetical protein	-
A9K65_RS33000	121841..122563	-	723	WP_064993491.1	SMC-Scp complex subunit ScpB	-
A9K65_RS33005	122566..123519	-	954	WP_064993490.1	DUF1403 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..531629	531629

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (2-68)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 70 a.a.
• Molecular weight: 7599.71 Da
• Isoelectric Point: 6.2481

>T75952 WP_086081393.1 NZ_CP021071:119187-119396 [Mesorhizobium sp. WSM1497]
MARISRVEDGNLGDAKFLDGIGELRIDYGPGYRIYFLKRGDVVVILLCGGDKGSQSRDIKRALDIANEV

Nucleotide

• Length: 210 bp

>T75952 NZ_CP021071:119187-119396 [Mesorhizobium sp. WSM1497]
ATGGCGCGCATCAGCCGCGTCGAGGACGGCAATCTCGGCGATGCCAAGTTCTTAGACGGCATCGGCGAACTTCGCATCGA
CTATGGACCGGGCTACCGCATTTACTTTCTCAAACGCGGCGACGTCGTCGTGATACTGCTGTGCGGCGGCGACAAGGGCA
GCCAGAGCCGCGACATCAAGCGCGCGCTGGACATCGCAAATGAGGTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10750.30 Da
• Isoelectric Point: 4.8227

>AT75952 WP_064993496.1 NZ_CP021071:119399-119701 [Mesorhizobium sp. WSM1497]
MAITTSKWDTADYLNTEEEIVAFLDAVMAEGDLQFLCKALGQVARSKGMTEIAKKTGMSRESLYKALSEGGNPSLGTVVS
VLDAMGLRLSVAPREHERAA

Nucleotide

• Length: 303 bp

>AT75952 NZ_CP021071:119399-119701 [Mesorhizobium sp. WSM1497]
ATGGCAATCACGACCAGCAAATGGGACACCGCCGACTACCTCAACACCGAAGAGGAGATCGTCGCCTTTCTTGACGCTGT
CATGGCGGAAGGCGACCTCCAGTTTCTGTGCAAGGCACTCGGCCAAGTCGCCCGATCCAAGGGCATGACGGAAATCGCCA
AGAAAACCGGCATGTCTCGCGAAAGCCTCTACAAGGCCCTCAGCGAGGGTGGGAACCCGTCCCTTGGCACGGTCGTGTCA
GTGCTCGACGCGATGGGGCTTCGCTTGTCAGTCGCCCCGCGCGAGCATGAGCGCGCTGCCTGA