Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3111977..3112758 Replicon chromosome
Accession NZ_CP021070
Organism Mesorhizobium sp. WSM1497

Toxin (Protein)


Gene name TacT2 Uniprot ID A0A3S2GFV8
Locus tag A9K65_RS15015 Protein ID WP_063168651.1
Coordinates 3111977..3112471 (-) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID E8T9Q9
Locus tag A9K65_RS15020 Protein ID WP_013530564.1
Coordinates 3112468..3112758 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A9K65_RS14985 3107245..3108051 - 807 WP_064994007.1 ABC transporter substrate-binding protein -
A9K65_RS14990 3108162..3109325 - 1164 WP_064994080.1 saccharopine dehydrogenase family protein -
A9K65_RS14995 3109322..3109822 - 501 WP_024501775.1 Lrp/AsnC family transcriptional regulator -
A9K65_RS15000 3109954..3110826 - 873 WP_064994079.1 LysR family transcriptional regulator -
A9K65_RS15005 3110964..3111170 + 207 WP_063168653.1 hypothetical protein -
A9K65_RS15010 3111192..3111941 + 750 WP_064994006.1 sulfite exporter TauE/SafE family protein -
A9K65_RS15015 3111977..3112471 - 495 WP_063168651.1 GNAT family N-acetyltransferase Toxin
A9K65_RS15020 3112468..3112758 - 291 WP_013530564.1 DUF1778 domain-containing protein Antitoxin
A9K65_RS15025 3112845..3113294 - 450 WP_027160859.1 universal stress protein -
A9K65_RS15030 3113503..3114084 + 582 WP_024501770.1 histidine phosphatase family protein -
A9K65_RS15035 3114115..3114516 - 402 WP_013530567.1 VOC family protein -
A9K65_RS15040 3114637..3115389 - 753 WP_064994005.1 rRNA pseudouridine synthase -
A9K65_RS15045 3115548..3115994 + 447 WP_064994004.1 SRPBCC domain-containing protein -
A9K65_RS15050 3116036..3116878 - 843 WP_063168648.1 helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(43-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 17511.18 Da        Isoelectric Point: 8.8832

>T75948 WP_063168651.1 NZ_CP021070:c3112471-3111977 [Mesorhizobium sp. WSM1497]
VTLSVPVPLADHHELGEFSSGITALDDWLKKRARTNQAGGASRTYVTCKGNRVIGYYAVASGAVKMVEASSRFRRNMPDP
IPVAVLGRLAVDQAYHGRGIGRALVRDAGLRILQAAEILGIKGIVVHAISDDARAFYITIGFEPSPVEPLTLMVTLADLS
ESIG

Download         Length: 495 bp

>T75948 NZ_CP021070:c3112471-3111977 [Mesorhizobium sp. WSM1497]
GTGACGCTGTCGGTCCCGGTTCCACTCGCGGATCATCACGAACTCGGGGAATTTTCGTCGGGCATTACAGCGCTCGACGA
TTGGTTGAAAAAACGTGCTCGCACCAATCAAGCGGGCGGTGCATCCCGGACCTATGTGACTTGCAAAGGCAACCGTGTGA
TTGGCTACTATGCCGTTGCCAGCGGCGCCGTGAAAATGGTCGAAGCTTCCAGCCGTTTCCGGCGCAACATGCCCGATCCG
ATCCCCGTCGCAGTGCTAGGCAGGTTAGCCGTAGACCAAGCCTATCACGGTCGGGGCATCGGTCGGGCGCTGGTAAGGGA
TGCCGGTCTACGAATTCTCCAGGCTGCTGAAATACTGGGCATCAAAGGCATAGTGGTGCATGCGATTTCCGACGATGCCC
GAGCTTTCTACATCACCATCGGGTTCGAGCCCTCGCCGGTCGAGCCGTTGACACTCATGGTCACGCTCGCTGATCTCAGC
GAAAGCATTGGCTGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10842.51 Da        Isoelectric Point: 9.9898

>AT75948 WP_013530564.1 NZ_CP021070:c3112758-3112468 [Mesorhizobium sp. WSM1497]
MPTSHHPVPKSVPLNIRVKPEVRNLIDRAAELLGKNRTDFMLEASQRAAEEALLNRLVFTVEPDVYAAFLARLDAPPQPN
DRLRRTMTTKAPWEAA

Download         Length: 291 bp

>AT75948 NZ_CP021070:c3112758-3112468 [Mesorhizobium sp. WSM1497]
ATGCCAACGTCACATCATCCGGTGCCGAAGAGCGTTCCGCTCAACATCCGCGTCAAGCCGGAAGTTCGCAATCTGATCGA
CCGCGCAGCCGAATTGCTCGGTAAGAATCGCACCGACTTCATGCTTGAAGCCTCGCAACGCGCCGCTGAGGAAGCGTTGC
TCAACCGTCTCGTGTTTACCGTCGAACCGGATGTCTACGCTGCGTTTCTGGCACGCCTCGACGCGCCGCCGCAGCCCAAC
GACCGATTGCGCCGCACGATGACGACAAAGGCTCCGTGGGAAGCTGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3S2GFV8

Antitoxin

Source ID Structure
AlphaFold DB A0A3S2IG73

References