Detailed information of TA system TA075946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 814746..815278
• Replicon: chromosome
• Accession: NZ_CP021069
• Organism: Burkholderia cenocepacia strain PC184 Mulks

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: B1JWG6
• Locus tag: B9Z07_RS20465
• Protein ID: WP_011544674.1
• Coordinates: 814955..815278 (+)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: B9Z07_RS20460
• Protein ID: WP_011544675.1
• Coordinates: 814746..814958 (+)
• Length: 71 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9Z07_RS20425	810206..811501	+	1296	WP_006476798.1	Tol-Pal system protein TolB	-
B9Z07_RS20430	811519..812031	+	513	WP_006484980.1	peptidoglycan-associated lipoprotein Pal	-
B9Z07_RS20435	812051..812800	+	750	WP_011544679.1	tol-pal system protein YbgF	-
B9Z07_RS20445	813232..813552	-	321	WP_041489160.1	helix-turn-helix domain-containing protein	-
B9Z07_RS20450	813691..814086	+	396	WP_175769797.1	hypothetical protein	-
B9Z07_RS20455	814154..814633	+	480	WP_105798309.1	hypothetical protein	-
B9Z07_RS20460	814746..814958	+	213	WP_011544675.1	antitoxin MazE family protein	Antitoxin
B9Z07_RS20465	814955..815278	+	324	WP_011544674.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9Z07_RS20470	815598..816122	+	525	WP_105798310.1	hypothetical protein	-
B9Z07_RS20475	816124..817158	+	1035	Protein_740	relaxase/mobilization nuclease domain-containing protein	-
B9Z07_RS20480	817281..817724	-	444	WP_034174243.1	YiaA/YiaB family protein	-
B9Z07_RS20485	817828..818775	-	948	WP_006476801.1	AraC family transcriptional regulator	-
B9Z07_RS20490	818905..819234	+	330	WP_006476802.1	carboxymuconolactone decarboxylase family protein	-
B9Z07_RS20495	819435..820268	+	834	WP_034174616.1	class II glutamine amidotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (7-67)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11668.77 Da
• Isoelectric Point: 8.9366

>T75946 WP_011544674.1 NZ_CP021069:814955-815278 [Burkholderia cenocepacia]
MNRGSIVTVALQGDFGKARPALVVQSDLFAEHPSVTLLLMSSSMVDAPLIRITVVPSEQNGLRMPSQIAVDKMFTVRREK
IGRPIGHLEDELMVAVNRSLLVFLGLA

Nucleotide

• Length: 324 bp

>T75946 NZ_CP021069:814955-815278 [Burkholderia cenocepacia]
ATGAATCGAGGGAGCATTGTCACAGTCGCGCTCCAAGGTGATTTCGGCAAGGCTCGGCCGGCGCTCGTGGTGCAGTCGGA
CCTGTTTGCCGAACATCCCAGTGTAACGTTGCTGCTAATGTCGAGCTCAATGGTCGATGCGCCCTTGATACGCATTACTG
TTGTCCCATCCGAGCAGAACGGCTTGCGCATGCCGTCCCAGATTGCCGTAGACAAAATGTTCACTGTGCGCCGCGAGAAA
ATCGGTCGCCCCATCGGCCACCTCGAAGATGAACTGATGGTTGCCGTCAATCGTTCGCTACTCGTTTTTCTAGGACTAGC
GTAA

Antitoxin

Protein

• Length: 71 a.a.
• Molecular weight: 8026.00 Da
• Isoelectric Point: 6.2095

>AT75946 WP_011544675.1 NZ_CP021069:814746-814958 [Burkholderia cenocepacia]
MAATTSERVRIHRENLRAAGLRPIQIWVPDVRRPGFADECARQSRMVHQSIDENDLLDFIEQATDLGHSQ

Nucleotide

• Length: 213 bp

>AT75946 NZ_CP021069:814746-814958 [Burkholderia cenocepacia]
ATGGCAGCTACCACTTCGGAGCGTGTTCGAATTCACCGCGAGAATCTTCGTGCGGCCGGGCTACGCCCAATTCAGATATG
GGTACCGGACGTCCGCCGGCCGGGTTTCGCCGATGAATGCGCTCGGCAATCGCGCATGGTCCACCAGAGCATCGACGAGA
ACGATCTGCTCGATTTCATTGAACAGGCTACGGACCTCGGACACTCACAATGA

Structures

Toxin

• AlphaFold DB: B1JWG6