Detailed information of TA system TA075936

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /SpoIISA(toxin)
• Location: 2258974..2259952
• Replicon: chromosome
• Accession: NZ_CP021061
• Organism: Bacillus thuringiensis strain ATCC 10792

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: M1Q940
• Locus tag: CAB88_RS12165
• Protein ID: WP_000624982.1
• Coordinates: 2258974..2259711 (+)
• Length: 246 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: M1PKC2
• Locus tag: CAB88_RS12170
• Protein ID: WP_000237819.1
• Coordinates: 2259824..2259952 (+)
• Length: 43 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CAB88_RS12140	2254616..2255398	+	783	WP_000355672.1	class I SAM-dependent methyltransferase	-
CAB88_RS12145	2255556..2257241	-	1686	WP_003270568.1	alpha-keto acid decarboxylase family protein	-
CAB88_RS12150	2257349..2257831	+	483	WP_000191889.1	MarR family winged helix-turn-helix transcriptional regulator	-
CAB88_RS12155	2257998..2258735	+	738	WP_000594152.1	2,3-diphosphoglycerate-dependent phosphoglycerate mutase	-
CAB88_RS12165	2258974..2259711	+	738	WP_000624982.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
CAB88_RS12170	2259824..2259952	+	129	WP_000237819.1	hypothetical protein	Antitoxin
CAB88_RS33870	2260025..2260201	+	177	WP_000852631.1	hypothetical protein	-
CAB88_RS12180	2260245..2260520	-	276	WP_001032898.1	hypothetical protein	-
CAB88_RS33875	2261031..2261207	+	177	WP_000852630.1	hypothetical protein	-
CAB88_RS12200	2261351..2262820	+	1470	WP_000287547.1	beta-Ala-His dipeptidase	-
CAB88_RS12205	2263066..2263875	+	810	WP_000678371.1	C39 family peptidase	-
CAB88_RS12210	2263901..2264503	+	603	WP_000517270.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (10-243)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 246 a.a.
• Molecular weight: 28323.03 Da
• Isoelectric Point: 8.2998

>T75936 WP_000624982.1 NZ_CP021061:2258974-2259711 [Bacillus thuringiensis]
MISNIRIGLFVLAIVFVVLVFFYWKNEELYEEKKQRIRKTWYGLFIISVTVYFMIKGIDLTLWKNLLMFTAMVIFVDIAF
ILTPNISEIWGAKFSDIGKTVQSIKRSLIASKARGEIYTTIIQNVNPSVFGTMEWHTEEEYTKSLNAFLDSYGEKIGAKI
VVFEAAKELNTNFRGIRSQFSIIVPLEHIEQLNEQKAVQVENVGIIPAKIVSDVFIVIDGKKNNLQDRDFENVYNLTIHH
SYFSK

Nucleotide

• Length: 738 bp

>T75936 NZ_CP021061:2258974-2259711 [Bacillus thuringiensis]
ATGATCTCTAACATTCGAATTGGCCTATTTGTTTTAGCGATTGTCTTTGTAGTCCTTGTTTTCTTTTATTGGAAAAATGA
GGAGTTGTACGAGGAGAAAAAGCAACGTATTAGAAAGACTTGGTATGGTTTGTTTATCATATCTGTCACCGTGTATTTCA
TGATAAAAGGAATTGATTTAACCCTCTGGAAAAATCTTTTAATGTTTACTGCAATGGTTATTTTTGTTGATATTGCGTTC
ATTTTAACACCTAATATTTCAGAAATATGGGGTGCGAAATTTAGTGATATTGGCAAGACAGTTCAATCAATAAAACGATC
ATTAATTGCATCTAAAGCGAGAGGAGAAATATACACGACAATTATTCAAAATGTTAATCCATCAGTATTCGGTACGATGG
AATGGCATACGGAAGAGGAATATACAAAGAGCTTAAATGCATTTTTAGATTCATATGGGGAAAAGATTGGTGCGAAAATT
GTTGTTTTTGAAGCCGCGAAGGAATTAAATACAAACTTTCGTGGTATTCGCTCGCAATTTAGTATTATCGTTCCGTTAGA
ACATATCGAGCAATTGAATGAGCAGAAAGCGGTGCAAGTAGAAAATGTCGGGATTATACCAGCAAAAATAGTAAGTGACG
TTTTCATTGTTATTGATGGAAAGAAAAATAACCTGCAAGATCGGGATTTTGAAAATGTATATAATTTAACAATACATCAT
AGTTATTTTAGTAAATAA

Antitoxin

Protein

• Length: 43 a.a.
• Molecular weight: 4953.36 Da
• Isoelectric Point: 3.9798

>AT75936 WP_000237819.1 NZ_CP021061:2259824-2259952 [Bacillus thuringiensis]
VVKDYDNDFFKEGNEDTTDFSLIKGDTLQQVEELEEELKRKS

Nucleotide

• Length: 129 bp

>AT75936 NZ_CP021061:2259824-2259952 [Bacillus thuringiensis]
GTGGTAAAAGATTATGACAATGATTTTTTTAAAGAAGGTAACGAAGATACAACAGATTTCTCTTTAATAAAAGGGGATAC
GTTGCAGCAAGTAGAAGAATTAGAAGAAGAATTAAAAAGAAAATCATAA

Structures

Toxin

• AlphaFold DB: M1Q940

Antitoxin

• AlphaFold DB: M1PKC2