Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 83715..84357 Replicon chromosome
Accession NZ_CP021061
Organism Bacillus thuringiensis strain ATCC 10792

Toxin (Protein)


Gene name pemK Uniprot ID R8I8B8
Locus tag CAB88_RS00510 Protein ID WP_000635965.1
Coordinates 84007..84357 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID R8I8H2
Locus tag CAB88_RS00505 Protein ID WP_000004570.1
Coordinates 83715..84002 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CAB88_RS00480 79032..79994 + 963 WP_000961144.1 UV DNA damage repair endonuclease UvsE -
CAB88_RS00485 79987..80559 - 573 WP_000908526.1 rhomboid family intramembrane serine protease -
CAB88_RS00490 80653..81012 + 360 WP_000583419.1 holo-ACP synthase -
CAB88_RS00495 81169..82119 + 951 WP_002094231.1 outer membrane lipoprotein carrier protein LolA -
CAB88_RS00500 82237..83406 + 1170 WP_000390593.1 alanine racemase -
CAB88_RS00505 83715..84002 + 288 WP_000004570.1 antitoxin EndoAI Antitoxin
CAB88_RS00510 84007..84357 + 351 WP_000635965.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
CAB88_RS00515 84426..86594 + 2169 WP_000426207.1 RNA-binding transcriptional accessory protein -
CAB88_RS00520 86652..86768 - 117 WP_001143640.1 cortex morphogenetic protein CmpA -
CAB88_RS00525 86964..87422 + 459 WP_000503730.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12948.04 Da        Isoelectric Point: 5.7168

>T75935 WP_000635965.1 NZ_CP021061:84007-84357 [Bacillus thuringiensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKKYGFERDSVILLEQI
RTIDKQRLTDKITHLDEVMMSRVDEALQISLGLIDF

Download         Length: 351 bp

>T75935 NZ_CP021061:84007-84357 [Bacillus thuringiensis]
TTGATTGTAAAACGCGGCGACGTGTATTTTGCAGACCTTTCCCCAGTTGTTGGTTCTGAGCAAGGAGGCGTTCGTCCGGT
TCTTGTCATTCAAAATGACATCGGAAATCGTTTTAGTCCAACGGTGATTGTAGCGGCTATTACTGCACAGATTCAAAAAG
CGAAATTACCCACTCATGTGGAAATTGATGCGAAAAAGTATGGTTTTGAGAGAGATTCTGTTATTTTACTTGAGCAGATT
CGAACAATCGATAAGCAACGCTTAACGGACAAAATCACTCATTTAGATGAAGTGATGATGAGTCGTGTAGATGAAGCGTT
ACAAATTAGTTTAGGACTAATAGATTTTTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10780.36 Da        Isoelectric Point: 8.9605

>AT75935 WP_000004570.1 NZ_CP021061:83715-84002 [Bacillus thuringiensis]
VSESSVTTEIVVRLPKQMVTELDGIGKQENKNRHELICQATQLLLRQHKTKKRYQHESMRRGYIEMGKINLGIASEAFLA
EYEAAHTVERLVSGG

Download         Length: 288 bp

>AT75935 NZ_CP021061:83715-84002 [Bacillus thuringiensis]
GTGTCCGAATCAAGTGTAACTACTGAAATCGTGGTTCGGTTGCCAAAGCAAATGGTAACGGAATTGGACGGAATTGGAAA
ACAAGAGAATAAGAATCGCCATGAACTAATTTGCCAGGCAACACAATTGTTATTGCGTCAACATAAGACGAAGAAACGCT
ACCAACATGAATCAATGCGACGTGGGTATATTGAAATGGGAAAAATTAATCTTGGTATTGCATCTGAAGCTTTCTTAGCG
GAGTATGAAGCAGCTCATACAGTAGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A366G118

Antitoxin

Source ID Structure
AlphaFold DB A0A366FY90

References