Detailed information of TA system TA075930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2028655..2029146
• Replicon: chromosome
• Accession: NZ_CP021058
• Organism: Macrococcus caseolyticus strain IMD0819

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CA207_RS10720
• Protein ID: WP_086039219.1
• Coordinates: 2028655..2029005 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: CA207_RS12250
• Protein ID: WP_101031708.1
• Coordinates: 2029006..2029146 (-)
• Length: 47 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CA207_RS10695	2023759..2025894	-	2136	WP_086039217.1	RNA-binding transcriptional accessory protein	-
CA207_RS10700	2025971..2026738	-	768	WP_015912228.1	RNA polymerase sigma factor SigB	-
CA207_RS10705	2026743..2027186	-	444	WP_015912229.1	anti-sigma B factor RsbW	-
CA207_RS10710	2027191..2027514	-	324	WP_015912230.1	anti-sigma factor antagonist	-
CA207_RS10715	2027599..2028606	-	1008	WP_086039218.1	PP2C family protein-serine/threonine phosphatase	-
CA207_RS10720	2028655..2029005	-	351	WP_086039219.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CA207_RS12250	2029006..2029146	-	141	WP_101031708.1	antitoxin MazE	Antitoxin
CA207_RS10725	2029207..2030349	-	1143	WP_086039220.1	alanine racemase	-
CA207_RS10730	2030375..2030731	-	357	WP_086039221.1	holo-ACP synthase	-
CA207_RS10735	2030673..2031194	-	522	WP_015912235.1	PH domain-containing protein	-
CA207_RS10740	2031178..2032689	-	1512	WP_086039222.1	PH domain-containing protein	-
CA207_RS10745	2032679..2033155	-	477	WP_041636191.1	PH domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-109)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12706.90 Da
• Isoelectric Point: 10.1643

>T75930 WP_086039219.1 NZ_CP021058:c2029005-2028655 [Macrococcus caseolyticus]
MKRGDVFLADLSPVKGSEQGGKRPVVIIQNNIGNKYSPTVIVAAITAKINKAKIPTHVEIGKATHRLEKDSVILLEQIRT
IDKNRLIDHLTVLDKAKMKEVDTALIISLGLSIDSE

Nucleotide

• Length: 351 bp

>T75930 NZ_CP021058:c2029005-2028655 [Macrococcus caseolyticus]
ATGAAACGCGGAGATGTTTTTTTGGCTGATCTGTCACCAGTGAAGGGATCTGAACAAGGCGGTAAACGCCCCGTCGTTAT
TATTCAGAATAATATAGGGAATAAATATAGTCCCACTGTGATTGTAGCGGCAATTACTGCAAAGATTAATAAGGCAAAAA
TCCCCACCCATGTTGAAATTGGCAAGGCAACGCATCGCTTAGAAAAGGATTCGGTAATACTGCTGGAACAAATTAGAACC
ATTGATAAGAATAGGTTGATTGATCATTTAACTGTGCTTGATAAGGCGAAGATGAAAGAAGTGGATACTGCACTTATAAT
TAGTTTAGGTTTATCAATCGATAGTGAATAG

Antitoxin

Protein

• Length: 47 a.a.
• Molecular weight: 5326.88 Da
• Isoelectric Point: 3.8301

>AT75930 WP_101031708.1 NZ_CP021058:c2029146-2029006 [Macrococcus caseolyticus]
MSQKLEQMLIEGYQSMAEINLSIASEHYVLECEVTDNHQTYTFDGE

Nucleotide

• Length: 141 bp

>AT75930 NZ_CP021058:c2029146-2029006 [Macrococcus caseolyticus]
ATGAGCCAGAAGCTAGAACAAATGCTGATAGAAGGTTATCAATCAATGGCAGAGATAAATTTATCGATAGCATCAGAGCA
TTATGTATTAGAATGCGAAGTTACTGATAACCATCAGACATATACCTTTGATGGTGAATAG