Detailed information of TA system TA075924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 4813582..4814177
• Replicon: chromosome
• Accession: NZ_CP021054
• Organism: Methylorubrum zatmanii strain PSBB041

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: B2G69_RS22235
• Protein ID: WP_056499989.1
• Coordinates: 4813582..4813872 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A1W6RNQ5
• Locus tag: B2G69_RS22240
• Protein ID: WP_056499991.1
• Coordinates: 4813875..4814177 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2G69_RS22215	4809637..4810593	-	957	WP_085857375.1	hypothetical protein	-
B2G69_RS22220	4810568..4811383	-	816	WP_085857376.1	ABC transporter ATP-binding protein	-
B2G69_RS22225	4811380..4812420	-	1041	WP_056499985.1	iron ABC transporter permease	-
B2G69_RS22230	4812417..4813250	-	834	WP_085857377.1	ABC transporter substrate-binding protein	-
B2G69_RS22235	4813582..4813872	+	291	WP_056499989.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B2G69_RS22240	4813875..4814177	+	303	WP_056499991.1	putative addiction module antidote protein	Antitoxin
B2G69_RS22245	4814199..4815764	-	1566	WP_003603047.1	glutamine-hydrolyzing GMP synthase	-
B2G69_RS22250	4815934..4816161	+	228	WP_003603049.1	hypothetical protein	-
B2G69_RS22255	4816347..4816958	-	612	WP_056499993.1	phosphatase PAP2 family protein	-
B2G69_RS22260	4817067..4818095	-	1029	WP_056118396.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Uncharacterized protein HI1420 (4-94)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10689.37 Da
• Isoelectric Point: 10.2219

>T75924 WP_056499989.1 NZ_CP021054:4813582-4813872 [Methylorubrum zatmanii]
MPTIRRTSVFQTWIDDLRDVRAVARIAKRIDRLALGNPGDVKPVGDGVSEMRIDYGPGYRIYFTAQGKQIVILLCGGDKS
SQERDIRAAKALAKEL

Nucleotide

• Length: 291 bp

>T75924 NZ_CP021054:4813582-4813872 [Methylorubrum zatmanii]
ATGCCGACGATCCGCCGTACGAGTGTCTTTCAGACTTGGATCGACGATTTGCGCGACGTTCGCGCCGTTGCGCGCATCGC
CAAGCGCATCGACCGGTTGGCGCTCGGCAATCCGGGCGACGTCAAACCGGTCGGAGACGGTGTCAGCGAGATGCGGATCG
ATTACGGCCCGGGCTACCGGATCTACTTCACCGCCCAAGGCAAGCAGATCGTCATTTTGCTTTGCGGCGGAGACAAGAGC
TCACAGGAGCGGGACATCCGAGCTGCGAAAGCGCTCGCGAAGGAGCTTTGA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10931.42 Da
• Isoelectric Point: 5.5368

>AT75924 WP_056499991.1 NZ_CP021054:4813875-4814177 [Methylorubrum zatmanii]
MALETQPYDSAEHLKTPEHIAAYLDAVLEDNDPALLAHALGVIARARGMTQIAQDVGRSREQLYRTLSDKGNPQLDTLMG
VLKSLGLRLSVHPVDVERSA

Nucleotide

• Length: 303 bp

>AT75924 NZ_CP021054:4813875-4814177 [Methylorubrum zatmanii]
ATGGCCCTCGAAACGCAGCCTTACGACAGCGCCGAGCACCTGAAGACGCCCGAACACATCGCGGCCTATCTCGATGCCGT
GTTGGAAGATAACGATCCCGCACTGCTCGCTCACGCGCTCGGTGTCATCGCGCGCGCCCGCGGAATGACGCAGATCGCTC
AGGACGTCGGCCGGTCGCGAGAACAACTCTACCGAACCCTGTCCGACAAAGGGAACCCGCAACTCGACACGCTCATGGGT
GTGCTGAAGTCGTTAGGATTGCGCCTCAGCGTGCATCCGGTCGACGTAGAGCGATCGGCCTGA

Structures

Antitoxin

• AlphaFold DB: A0A1W6RNQ5