Detailed information of TA system TA075921

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-dinJ/YafQ-RelB
• Location: 318458..318989
• Replicon: chromosome
• Accession: NZ_CP021054
• Organism: Methylorubrum zatmanii strain PSBB041

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: B2G69_RS01430
• Protein ID: WP_003605878.1
• Coordinates: 318708..318989 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: dinJ
• Uniprot ID: C5B2A4
• Locus tag: B2G69_RS01425
• Protein ID: WP_003605879.1
• Coordinates: 318458..318721 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B2G69_RS01405	314499..315125	+	627	WP_056153177.1	bifunctional nicotinamidase/pyrazinamidase	-
B2G69_RS01410	315295..316152	-	858	WP_056501120.1	aminotransferase class IV	-
B2G69_RS01415	316139..316768	-	630	WP_056501123.1	aminodeoxychorismate/anthranilate synthase component II	-
B2G69_RS01420	316765..318165	-	1401	WP_056501127.1	aminodeoxychorismate synthase component I	-
B2G69_RS01425	318458..318721	+	264	WP_003605879.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
B2G69_RS01430	318708..318989	+	282	WP_003605878.1	type II toxin-antitoxin system YafQ family toxin	Toxin
B2G69_RS01435	319106..320452	+	1347	WP_056501131.1	30S ribosomal protein S12 methylthiotransferase RimO	-
B2G69_RS01440	320549..320734	+	186	WP_003605874.1	hypothetical protein	-
B2G69_RS01445	320741..321163	+	423	WP_085856230.1	DoxX family protein	-
B2G69_RS01450	321252..321443	+	192	WP_085856231.1	hypothetical protein	-
B2G69_RS01455	321581..322411	-	831	WP_085856232.1	nucleoside triphosphate pyrophosphohydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, YafQ-like toxin (2-91)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-83)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10664.24 Da
• Isoelectric Point: 10.2159

>T75921 WP_003605878.1 NZ_CP021054:318708-318989 [Methylorubrum zatmanii]
MRTIERTTQFKRDYKREMKGRHRASLQADLTAVLTELAADRPLAARLRDHALTGNWADHRDCHVKPDLVLIYRLAGTETL
QLVRLGSHAELGF

Nucleotide

• Length: 282 bp

>T75921 NZ_CP021054:318708-318989 [Methylorubrum zatmanii]
ATGCGGACGATTGAGCGAACCACGCAGTTCAAGCGTGACTACAAGCGGGAGATGAAGGGCAGGCACCGGGCGAGCCTTCA
AGCCGACCTGACGGCGGTGCTGACCGAGTTGGCCGCGGATCGCCCCCTCGCGGCGCGCCTGCGTGACCACGCCCTGACCG
GCAACTGGGCCGACCATCGCGACTGCCACGTGAAGCCGGATCTGGTGCTGATCTATCGGCTTGCAGGAACGGAAACGCTC
CAATTGGTGCGCCTCGGCTCGCACGCCGAACTGGGCTTCTGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 9356.81 Da
• Isoelectric Point: 4.4230

>AT75921 WP_003605879.1 NZ_CP021054:318458-318721 [Methylorubrum zatmanii]
MPTKSLIQARIDADTKEEATAVLKAMGLTVSDAVRMMLVRVAREKALPFEPLVPNETTIAAIREARSGNLPGFTSVEALM
ADLDADD

Nucleotide

• Length: 264 bp

>AT75921 NZ_CP021054:318458-318721 [Methylorubrum zatmanii]
ATGCCGACCAAGTCGCTGATCCAGGCGCGGATCGATGCCGACACCAAGGAAGAGGCCACAGCCGTTCTCAAGGCCATGGG
GCTGACCGTCTCCGATGCGGTTCGCATGATGCTCGTGCGCGTCGCGCGAGAGAAGGCGCTTCCCTTCGAGCCTCTGGTTC
CCAACGAAACGACCATCGCAGCCATCCGCGAGGCGCGGTCCGGTAACCTTCCGGGCTTCACCAGCGTCGAGGCGCTGATG
GCCGATCTCGATGCGGACGATTGA

Structures

Antitoxin

• AlphaFold DB: C5B2A4