Detailed information of TA system TA075903

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 39679..40220
• Replicon: plasmid pG
• Accession: NZ_CP021022
• Organism: Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6167

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XcfCFBP6167P_RS24395
• Protein ID: WP_007968778.1
• Coordinates: 39679..40005 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7W9EDN7
• Locus tag: XcfCFBP6167P_RS24400
• Protein ID: WP_033478260.1
• Coordinates: 40002..40220 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XcfCFBP6167P_RS24380	34845..35042	+	198	WP_019303588.1	hypothetical protein	-
XcfCFBP6167P_RS24385	35046..36620	+	1575	WP_019303587.1	type IV secretion system DNA-binding domain-containing protein	-
XcfCFBP6167P_RS24390	36634..39609	+	2976	WP_099868349.1	conjugative relaxase	-
XcfCFBP6167P_RS24395	39679..40005	-	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XcfCFBP6167P_RS24400	40002..40220	-	219	WP_033478260.1	antitoxin MazE family protein	Antitoxin
XcfCFBP6167P_RS24405	40357..40773	-	417	WP_017159868.1	hypothetical protein	-
XcfCFBP6167P_RS24410	40827..41241	-	415	Protein_54	hypothetical protein	-
XcfCFBP6167P_RS24415	41252..41825	-	574	Protein_55	phospholipase D family protein	-
XcfCFBP6167P_RS24420	41855..42067	-	213	WP_099868351.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42380	42380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T75903 WP_007968778.1 NZ_CP021022:c40005-39679 [Xanthomonas citri pv. phaseoli var. fuscans]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T75903 NZ_CP021022:c40005-39679 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8476.58 Da
• Isoelectric Point: 5.0742

>AT75903 WP_033478260.1 NZ_CP021022:c40220-40002 [Xanthomonas citri pv. phaseoli var. fuscans]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT75903 NZ_CP021022:c40220-40002 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7W9EDN7