Detailed information of TA system TA075902

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 25784..26319
• Replicon: plasmid pG
• Accession: NZ_CP021022
• Organism: Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6167

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: Q3C0B3
• Locus tag: XcfCFBP6167P_RS24305
• Protein ID: WP_007974210.1
• Coordinates: 25784..26104 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: Q3C0B2
• Locus tag: XcfCFBP6167P_RS24310
• Protein ID: WP_011345582.1
• Coordinates: 26104..26319 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XcfCFBP6167P_RS24275	21980..22279	-	300	WP_041854933.1	VirB3 family type IV secretion system protein	-
XcfCFBP6167P_RS24280	22276..22653	-	378	WP_011345609.1	TrbC/VirB2 family protein	-
XcfCFBP6167P_RS24285	22682..22963	-	282	WP_041854934.1	hypothetical protein	-
XcfCFBP6167P_RS24295	23467..23796	+	330	WP_007967027.1	hypothetical protein	-
XcfCFBP6167P_RS24300	24297..25115	+	819	WP_011345581.1	hypothetical protein	-
XcfCFBP6167P_RS24305	25784..26104	-	321	WP_007974210.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XcfCFBP6167P_RS24310	26104..26319	-	216	WP_011345582.1	antitoxin MazE family protein	Antitoxin
XcfCFBP6167P_RS24315	26565..26891	-	327	WP_007974207.1	hypothetical protein	-
XcfCFBP6167P_RS24320	26888..27514	-	627	WP_007974205.1	ParA family protein	-
XcfCFBP6167P_RS24325	27990..28676	+	687	WP_007968793.1	DNA-binding protein	-
XcfCFBP6167P_RS24330	28835..29797	+	963	WP_011345584.1	recombinase family protein	-
XcfCFBP6167P_RS24335	29884..30114	+	231	WP_046935364.1	hypothetical protein	-
XcfCFBP6167P_RS24340	30498..30854	-	357	WP_041854930.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42380	42380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-102)

Antitoxin

• Antitoxin MazE-like (7-70)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11571.48 Da
• Isoelectric Point: 6.2890

>T75902 WP_007974210.1 NZ_CP021022:c26104-25784 [Xanthomonas citri pv. phaseoli var. fuscans]
MQRGDLVTVSLQGDYGKPRPALVVQSDLLTDLESVVLCPVTSDLRNAAFRVTVEPNPANGLRALSQVMVDKLSTLPRTKI
SEPFGRLDDERMKAIDRALLLVVGVI

Nucleotide

• Length: 321 bp

>T75902 NZ_CP021022:c26104-25784 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGCAGCGGGGCGACCTTGTGACGGTTTCATTGCAGGGCGACTATGGCAAACCACGGCCCGCCTTGGTCGTTCAATCCGA
CTTGCTCACCGACCTGGAAAGCGTCGTGCTGTGCCCGGTGACAAGCGACCTGCGCAATGCCGCGTTCCGCGTCACCGTGG
AACCGAACCCGGCCAACGGGCTGCGCGCGCTCTCGCAGGTCATGGTGGACAAGCTCTCAACGCTGCCTCGCACCAAGATC
AGCGAACCGTTTGGCCGCCTCGATGATGAGCGTATGAAAGCGATAGACAGGGCGCTCCTGCTGGTCGTTGGCGTTATCTG
A

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 8190.20 Da
• Isoelectric Point: 7.2801

>AT75902 WP_011345582.1 NZ_CP021022:c26319-26104 [Xanthomonas citri pv. phaseoli var. fuscans]
MSSIHESKVARHRERMRAAGLRPVQFWVPDTRSPEFAAQVRKQCQNLKGDPAETEVLRFTEEAATHVEGWE

Nucleotide

• Length: 216 bp

>AT75902 NZ_CP021022:c26319-26104 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGTCATCAATTCACGAATCAAAGGTAGCCCGGCACCGCGAGCGGATGCGCGCTGCGGGGCTACGGCCTGTCCAGTTTTG
GGTGCCCGACACACGCTCTCCCGAGTTCGCGGCGCAAGTGCGCAAGCAGTGCCAAAACCTGAAGGGCGACCCGGCAGAAA
CCGAGGTTTTGCGCTTCACCGAAGAGGCCGCAACGCACGTTGAGGGCTGGGAGTGA

Structures

Toxin

• AlphaFold DB: Q3C0B3

Antitoxin

• AlphaFold DB: Q3C0B2