Detailed information of TA system TA075901

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 9688..10229
• Replicon: plasmid pG
• Accession: NZ_CP021022
• Organism: Xanthomonas citri pv. phaseoli var. fuscans strain CFBP6167

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: XcfCFBP6167P_RS24195
• Protein ID: WP_007968778.1
• Coordinates: 9688..10014 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A7W9EDN7
• Locus tag: XcfCFBP6167P_RS24200
• Protein ID: WP_033478260.1
• Coordinates: 10011..10229 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XcfCFBP6167P_RS24180	4854..5051	+	198	WP_019303588.1	hypothetical protein	-
XcfCFBP6167P_RS24185	5055..6629	+	1575	WP_019303587.1	type IV secretion system DNA-binding domain-containing protein	-
XcfCFBP6167P_RS24190	6643..9618	+	2976	WP_099868349.1	conjugative relaxase	-
XcfCFBP6167P_RS24195	9688..10014	-	327	WP_007968778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
XcfCFBP6167P_RS24200	10011..10229	-	219	WP_033478260.1	antitoxin MazE family protein	Antitoxin
XcfCFBP6167P_RS24205	10366..10782	-	417	WP_017159868.1	hypothetical protein	-
XcfCFBP6167P_RS24210	10836..11249	-	414	WP_041854931.1	hypothetical protein	-
XcfCFBP6167P_RS24215	11260..11835	-	576	WP_041854932.1	phospholipase D family protein	-
XcfCFBP6167P_RS24220	11878..12465	-	588	WP_011345600.1	hypothetical protein	-
XcfCFBP6167P_RS24225	12511..13398	-	888	WP_041854937.1	lytic transglycosylase domain-containing protein	-
XcfCFBP6167P_RS24230	13409..14431	-	1023	WP_007968768.1	P-type DNA transfer ATPase VirB11	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..42380	42380

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11732.81 Da
• Isoelectric Point: 9.2550

>T75901 WP_007968778.1 NZ_CP021022:c10014-9688 [Xanthomonas citri pv. phaseoli var. fuscans]
MMRGDFVTIAMQGDFGKPRPALVIQANQFDEHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLLKPSQVMVDKAMTVKRDK
IGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T75901 NZ_CP021022:c10014-9688 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGATGCGAGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCACGGCCCGCTCTGGTCATCCAGGCCAA
CCAGTTTGACGAACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTTGTTGCTGCGCCGCTGCTGCGCATTACCG
TTCATCCAAGTACCGACAATGGCCTGCTGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
ATAGGTCGGGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8476.58 Da
• Isoelectric Point: 5.0742

>AT75901 WP_033478260.1 NZ_CP021022:c10229-10011 [Xanthomonas citri pv. phaseoli var. fuscans]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMEDTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT75901 NZ_CP021022:c10229-10011 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGTGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGAGGACA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A7W9EDN7