Detailed information of TA system TA075887

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 3155455..3156372
• Replicon: chromosome
• Accession: NZ_CP021011
• Organism: Bacillus velezensis strain GFP-2

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: -
• Locus tag: B6257_RS16440
• Protein ID: WP_057079950.1
• Coordinates: 3155626..3156372 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: I2HQ14
• Locus tag: B6257_RS16435
• Protein ID: WP_003154807.1
• Coordinates: 3155455..3155625 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6257_RS16390	3150608..3151120	+	513	WP_101562928.1	sigma-70 family RNA polymerase sigma factor	-
B6257_RS16395	3151233..3151457	+	225	Protein_3122	terminase	-
B6257_RS16400	3151477..3152310	+	834	Protein_3123	hypothetical protein	-
B6257_RS16405	3152323..3152694	+	372	WP_070081681.1	hypothetical protein	-
B6257_RS16410	3152699..3152896	+	198	WP_071391956.1	XkdX family protein	-
B6257_RS16415	3152953..3153714	+	762	WP_071391955.1	phage portal protein	-
B6257_RS16420	3153766..3154029	+	264	WP_101562929.1	hemolysin XhlA family protein	-
B6257_RS16425	3154043..3154306	+	264	WP_003154813.1	phage holin	-
B6257_RS16430	3154320..3155198	+	879	WP_024085195.1	N-acetylmuramoyl-L-alanine amidase	-
B6257_RS16435	3155455..3155625	-	171	WP_003154807.1	type II toxin-antitoxin system SpoIISB family antitoxin	Antitoxin
B6257_RS16440	3155626..3156372	-	747	WP_057079950.1	type II toxin-antitoxin system SpoIISA family toxin	Toxin
B6257_RS16445	3156477..3157475	-	999	WP_003154805.1	inorganic phosphate transporter	-
B6257_RS16450	3157488..3158105	-	618	WP_003154804.1	DUF47 domain-containing protein	-
B6257_RS16455	3158391..3159707	-	1317	WP_101562930.1	amino acid permease	-
B6257_RS16460	3160030..3160980	+	951	WP_077722102.1	ring-cleaving dioxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3142325..3164549	22224

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-239)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29033.52 Da
• Isoelectric Point: 4.7755

>T75887 WP_057079950.1 NZ_CP021011:c3156372-3155626 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVVGAMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYGEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Nucleotide

• Length: 747 bp

>T75887 NZ_CP021011:c3156372-3155626 [Bacillus velezensis]
ATGCTTTTGTTTTTTCAGATCATGGTGTGGACGATGGCGGCCGCACTCATTTTATATGTGTACGCATCCTGGCGGTATGA
GGCAAAGGTAAAAGAAAAAATGTTTGCCATCCGTAAAACGTGGTATTTACTATTTGTAGTAGGCGCCATGGTCTATTGGA
CATATGATCCCGAATCGCTCTTTGCCGCATGGAGACAATATTTGATTGTTGCCGTCTGCTTTGCCCTTATTGATGCGTTT
ATCTTTTTGAGCGCGTATATCAAAAAGCTCGCCGGCAATGAATTAGAGACGGATACGCGGGAAATTTTAGAAGAAAATAA
CGAAATGCTTCACTCCTATCTTGAAAAACTGAAAACCTATCAATATCTGTTGAAAAATGAACCGATCCATGTATATTATG
GAAGTACAGAAGCTTATGCGGAAGGCATCAGCAGACTGCTGGCAGCTTATGGAGAAAAAATGAATGTCACGGCTTCCTTG
TGCGACTATTCCGCACAATCGGATAAGGACCGCTTGACGGAGCATATGCCTGATGCGGCGGACGTTCAGTCCCGTCTTAA
CCGTAAAGACGTCTACTATGATCAAAAAGGCAGGCTTGTGCTTATTCCGTTTACAGTCCAGAACCGCCATTACGTGATCA
AACTCACGTCGGAAAACCTGTTGACGGAATTCGATTATTTGCTTTTCACATCGCTAACGAGCATTTATGATTTAATGCTG
CCAATAGAAGAGGAAGGTGACGGTTAA

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6676.74 Da
• Isoelectric Point: 10.9273

>AT75887 WP_003154807.1 NZ_CP021011:c3155625-3155455 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Nucleotide

• Length: 171 bp

>AT75887 NZ_CP021011:c3155625-3155455 [Bacillus velezensis]
ATGGAGCAGGCATTTCAAAACGGGCAGCCGGAACAAAGAGCGAAACCGTTCAAAATGTTTAAAAAACGTTCAATGACAAG
TATCGCAAGTTACCAGGTCAGCCCTCATACCGCCAGAATTTTCAAAGAAAACGAGCGGCTGATTGACAAGTATAAACAAA
AAAAAGCTTGA

Structures

Antitoxin

• AlphaFold DB: I2HQ14