Detailed information of TA system TA075886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2431973..2432610
• Replicon: chromosome
• Accession: NZ_CP021011
• Organism: Bacillus velezensis strain GFP-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: B6257_RS12545
• Protein ID: WP_003156187.1
• Coordinates: 2432260..2432610 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: I2HMS5
• Locus tag: B6257_RS12540
• Protein ID: WP_003156188.1
• Coordinates: 2431973..2432254 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B6257_RS12520	2428338..2428937	-	600	WP_003156193.1	rhomboid family intramembrane serine protease	-
B6257_RS12525	2429030..2429395	+	366	WP_071392104.1	holo-ACP synthase	-
B6257_RS12530	2429560..2430567	+	1008	WP_003156191.1	outer membrane lipoprotein carrier protein LolA	-
B6257_RS12535	2430684..2431853	+	1170	WP_101562726.1	alanine racemase	-
B6257_RS12540	2431973..2432254	+	282	WP_003156188.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
B6257_RS12545	2432260..2432610	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
B6257_RS12550	2432728..2433549	+	822	WP_003156182.1	STAS domain-containing protein	-
B6257_RS12555	2433554..2433919	+	366	WP_003156180.1	RsbT antagonist protein RsbS	-
B6257_RS12560	2433922..2434323	+	402	WP_003156178.1	anti-sigma regulatory factor	-
B6257_RS12565	2434335..2435342	+	1008	WP_003156177.1	PP2C family protein-serine/threonine phosphatase	-
B6257_RS12570	2435406..2435735	+	330	WP_003156176.1	anti-sigma factor antagonist RsbV	-
B6257_RS12575	2435732..2436214	+	483	WP_003156173.1	anti-sigma B factor RsbW	-
B6257_RS12580	2436180..2436968	+	789	WP_003156171.1	RNA polymerase sigma factor SigB	-
B6257_RS12585	2436968..2437570	+	603	WP_003156170.1	SpoIIE family protein phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T75886 WP_003156187.1 NZ_CP021011:2432260-2432610 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T75886 NZ_CP021011:2432260-2432610 [Bacillus velezensis]
TTGATTGTAAAGCGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGCGTACGCCCGGT
TTTAGTGATCCAGAACGATATCGGAAATCGCTTTAGCCCGACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATTGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGATTAACGGATAAAATAACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCCT
GCAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10562.92 Da
• Isoelectric Point: 6.4706

>AT75886 WP_003156188.1 NZ_CP021011:2431973-2432254 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT75886 NZ_CP021011:2431973-2432254 [Bacillus velezensis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACTTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGGACAACTCGCCATAACC
GTGATTTGATGAGACGCGGGTATATGGAAATGGCGAAAATCAACTTGAATATCTCTTCTGAGGCTCACTATGCTGAGTGC
GAAGCAGAAACGACCGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: I2HMS5