Detailed information of TA system TA075875

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 11479..12146
• Replicon: plasmid pA
• Accession: NZ_CP020993
• Organism: Xanthomonas citri pv. phaseoli var. fuscans strain CFBP4885

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3T0G501
• Locus tag: XcfCFBP4885P_RS22430
• Protein ID: WP_022557732.1
• Coordinates: 11730..12146 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U4M4Z6
• Locus tag: XcfCFBP4885P_RS22425
• Protein ID: WP_022557731.1
• Coordinates: 11479..11733 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XcfCFBP4885P_RS22400	6737..7651	-	915	WP_022557729.1	hypothetical protein	-
XcfCFBP4885P_RS23880	8334..8438	+	105	Protein_10	type III secretion system effector protein	-
XcfCFBP4885P_RS22410	8573..9625	+	1053	WP_022557730.1	type III secretion system YopJ family effector AvrBsT	-
XcfCFBP4885P_RS22415	9759..10985	+	1227	WP_011345585.1	IS256-like element ISXax1 family transposase	-
XcfCFBP4885P_RS22420	11022..11267	-	246	Protein_13	recombinase family protein	-
XcfCFBP4885P_RS22425	11479..11733	+	255	WP_022557731.1	plasmid stability protein	Antitoxin
XcfCFBP4885P_RS22430	11730..12146	+	417	WP_022557732.1	type II toxin-antitoxin system VapC family toxin	Toxin
XcfCFBP4885P_RS22435	12160..12714	+	555	WP_033482006.1	plasmid pRiA4b ORF-3 family protein	-
XcfCFBP4885P_RS22440	12789..12926	-	138	Protein_17	transposase	-
XcfCFBP4885P_RS22445	12996..13526	-	531	Protein_18	DNA topoisomerase III	-
XcfCFBP4885P_RS22450	13678..14970	-	1293	WP_033481893.1	hypothetical protein	-
XcfCFBP4885P_RS22455	15097..16416	-	1320	WP_022557736.1	DUF4990 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..54803	54803
	-	inside	IScluster/Tn	-	-	9759..12926	3167

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14833.04 Da
• Isoelectric Point: 4.6812

>T75875 WP_022557732.1 NZ_CP020993:11730-12146 [Xanthomonas citri pv. phaseoli var. fuscans]
MIVLDTNVVSEAMKPEPHPSVRTWLNDQAAETLYLSSVTLAELLFGIAALPAGKRKDMLEQALDGLMGLFRDRVLPFDID
AARRYAELAVTAKIGGRGFPTPDGYIAAIAASRGFIVASRDTAPYEAAGVTVINPWEG

Nucleotide

• Length: 417 bp

>T75875 NZ_CP020993:11730-12146 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9388.80 Da
• Isoelectric Point: 8.9326

>AT75875 WP_022557731.1 NZ_CP020993:11479-11733 [Xanthomonas citri pv. phaseoli var. fuscans]
MAMLTVRNLPDDVHRALRMRAAQNGHSTEAEVREILATAVKPETRVRLGEALAALGRKIGLTNEDFEVFDRVRDKTPAVP
PRLE

Nucleotide

• Length: 255 bp

>AT75875 NZ_CP020993:11479-11733 [Xanthomonas citri pv. phaseoli var. fuscans]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A3T0G501

Antitoxin

• AlphaFold DB: A0A3T0G545