Detailed information of TA system TA075844

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 39074..39741
• Replicon: plasmid pA
• Accession: NZ_CP020972
• Organism: Xanthomonas phaseoli pv. phaseoli strain CFBP6546R

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3T0G501
• Locus tag: XppCFBP6546P_RS22050
• Protein ID: WP_022557732.1
• Coordinates: 39074..39490 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U4M4Z6
• Locus tag: XppCFBP6546P_RS22055
• Protein ID: WP_022557731.1
• Coordinates: 39487..39741 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XppCFBP6546P_RS22025 (XppCFBP6546P_22870)	34804..36123	+	1320	WP_022557736.1	right-handed parallel beta-helix repeat-containing protein	-
XppCFBP6546P_RS22030 (XppCFBP6546P_22875)	36250..37542	+	1293	WP_033481893.1	M12 family metallo-peptidase	-
XppCFBP6546P_RS22035 (XppCFBP6546P_22880)	37697..38245	+	549	WP_022557734.1	DNA topoisomerase	-
XppCFBP6546P_RS22040 (XppCFBP6546P_22885)	38294..38431	+	138	Protein_45	transposase	-
XppCFBP6546P_RS22045 (XppCFBP6546P_22890)	38506..39060	-	555	WP_033482006.1	plasmid pRiA4b ORF-3 family protein	-
XppCFBP6546P_RS22050 (XppCFBP6546P_22895)	39074..39490	-	417	WP_022557732.1	type II toxin-antitoxin system VapC family toxin	Toxin
XppCFBP6546P_RS22055 (XppCFBP6546P_22900)	39487..39741	-	255	WP_022557731.1	plasmid stability protein	Antitoxin
XppCFBP6546P_RS22060 (XppCFBP6546P_22905)	39953..40546	+	594	WP_022560487.1	recombinase family protein	-
XppCFBP6546P_RS22065 (XppCFBP6546P_22910)	40543..43572	+	3030	WP_022560486.1	Tn3 family transposase	-
XppCFBP6546P_RS22070 (XppCFBP6546P_24170)	43605..43990	+	386	Protein_51	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virB1	1..60283	60283
	-	inside	IScluster/Tn	-	virB1	38294..55871	17577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14833.04 Da
• Isoelectric Point: 4.6812

>T75844 WP_022557732.1 NZ_CP020972:c39490-39074 [Xanthomonas phaseoli pv. phaseoli]
MIVLDTNVVSEAMKPEPHPSVRTWLNDQAAETLYLSSVTLAELLFGIAALPAGKRKDMLEQALDGLMGLFRDRVLPFDID
AARRYAELAVTAKIGGRGFPTPDGYIAAIAASRGFIVASRDTAPYEAAGVTVINPWEG

Nucleotide

• Length: 417 bp

>T75844 NZ_CP020972:c39490-39074 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9388.80 Da
• Isoelectric Point: 8.9326

>AT75844 WP_022557731.1 NZ_CP020972:c39741-39487 [Xanthomonas phaseoli pv. phaseoli]
MAMLTVRNLPDDVHRALRMRAAQNGHSTEAEVREILATAVKPETRVRLGEALAALGRKIGLTNEDFEVFDRVRDKTPAVP
PRLE

Nucleotide

• Length: 255 bp

>AT75844 NZ_CP020972:c39741-39487 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A3T0G501

Antitoxin

• AlphaFold DB: A0A3T0G545