Detailed information of TA system TA075837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 49749..50416
• Replicon: plasmid pA
• Accession: NZ_CP020965
• Organism: Xanthomonas phaseoli pv. phaseoli strain CFBP412

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A3T0G501
• Locus tag: XppCFBP412P_RS23200
• Protein ID: WP_022557732.1
• Coordinates: 49749..50165 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U4M4Z6
• Locus tag: XppCFBP412P_RS23205
• Protein ID: WP_022557731.1
• Coordinates: 50162..50416 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
XppCFBP412P_RS23150	45333..45527	-	195	WP_080948835.1	ribbon-helix-helix domain-containing protein	-
XppCFBP412P_RS23165	46184..46507	+	324	WP_039572152.1	hypothetical protein	-
XppCFBP412P_RS23175	46911..47186	+	276	WP_039572155.1	hypothetical protein	-
XppCFBP412P_RS23835	47207..47497	+	291	WP_127446651.1	hypothetical protein	-
XppCFBP412P_RS23180	47853..48251	+	399	WP_039572158.1	hypothetical protein	-
XppCFBP412P_RS23840	48324..48485	+	162	WP_157926053.1	hypothetical protein	-
XppCFBP412P_RS23185	48482..48721	+	240	WP_039572160.1	hypothetical protein	-
XppCFBP412P_RS23190	48735..49100	+	366	Protein_50	hypothetical protein	-
XppCFBP412P_RS23195	49181..49735	-	555	WP_033482006.1	plasmid pRiA4b ORF-3 family protein	-
XppCFBP412P_RS23200	49749..50165	-	417	WP_022557732.1	type II toxin-antitoxin system VapC family toxin	Toxin
XppCFBP412P_RS23205	50162..50416	-	255	WP_022557731.1	plasmid stability protein	Antitoxin
XppCFBP412P_RS23210	50628..51221	+	594	WP_022560487.1	recombinase family protein	-
XppCFBP412P_RS23215	51218..54247	+	3030	WP_022560486.1	Tn3 family transposase	-
XppCFBP412P_RS23220	54280..54510	+	231	Protein_56	hypothetical protein	-
XppCFBP412P_RS23225	54608..54952	+	345	WP_039572167.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	virB1	1..66609	66609

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 14833.04 Da
• Isoelectric Point: 4.6812

>T75837 WP_022557732.1 NZ_CP020965:c50165-49749 [Xanthomonas phaseoli pv. phaseoli]
MIVLDTNVVSEAMKPEPHPSVRTWLNDQAAETLYLSSVTLAELLFGIAALPAGKRKDMLEQALDGLMGLFRDRVLPFDID
AARRYAELAVTAKIGGRGFPTPDGYIAAIAASRGFIVASRDTAPYEAAGVTVINPWEG

Nucleotide

• Length: 417 bp

>T75837 NZ_CP020965:c50165-49749 [Xanthomonas phaseoli pv. phaseoli]
ATGATCGTTCTCGATACCAACGTCGTTTCCGAGGCGATGAAGCCCGAGCCGCACCCATCCGTGCGGACCTGGTTGAACGA
TCAAGCCGCTGAAACACTGTACCTGTCCAGCGTGACGCTGGCCGAACTGCTATTTGGCATCGCTGCGCTTCCTGCCGGTA
AGCGCAAGGACATGCTGGAACAGGCCCTTGATGGCCTCATGGGCCTGTTTAGGGATCGGGTGTTGCCATTCGACATCGAT
GCAGCGCGGCGTTATGCCGAATTGGCCGTGACGGCAAAGATCGGAGGGCGAGGATTTCCGACACCCGACGGCTACATCGC
AGCGATTGCTGCCTCGCGGGGTTTCATTGTGGCGTCACGCGACACGGCCCCTTACGAAGCGGCCGGCGTAACCGTCATCA
ATCCGTGGGAAGGGTGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9388.80 Da
• Isoelectric Point: 8.9326

>AT75837 WP_022557731.1 NZ_CP020965:c50416-50162 [Xanthomonas phaseoli pv. phaseoli]
MAMLTVRNLPDDVHRALRMRAAQNGHSTEAEVREILATAVKPETRVRLGEALAALGRKIGLTNEDFEVFDRVRDKTPAVP
PRLE

Nucleotide

• Length: 255 bp

>AT75837 NZ_CP020965:c50416-50162 [Xanthomonas phaseoli pv. phaseoli]
ATGGCAATGCTGACAGTACGTAATCTGCCGGATGATGTGCACCGCGCATTGCGCATGCGGGCCGCTCAAAATGGGCACAG
CACCGAGGCCGAGGTCCGCGAGATTCTGGCGACCGCAGTCAAGCCAGAGACGCGCGTTCGCCTAGGCGAAGCCTTGGCGG
CTTTGGGTCGCAAGATTGGCCTGACGAATGAGGACTTCGAGGTCTTCGATCGGGTAAGAGACAAGACGCCGGCCGTCCCG
CCGAGGCTTGAATGA

Structures

Toxin

• AlphaFold DB: A0A3T0G501

Antitoxin

• AlphaFold DB: A0A3T0G545