Detailed information of TA system TA075809

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2076370..2076899
• Replicon: chromosome
• Accession: NZ_CP020959
• Organism: Staphylococcus aureus strain H489

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B9T45_RS10795
• Protein ID: WP_000621175.1
• Coordinates: 2076370..2076732 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B9T45_RS10800
• Protein ID: WP_000948331.1
• Coordinates: 2076729..2076899 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9T45_RS10770	2073348..2074118	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
B9T45_RS10775	2074093..2074572	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
B9T45_RS10780	2074574..2074900	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B9T45_RS10785	2075019..2076020	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B9T45_RS10795	2076370..2076732	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9T45_RS10800	2076729..2076899	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B9T45_RS10805	2076984..2078132	-	1149	WP_001281145.1	alanine racemase	-
B9T45_RS10810	2078198..2078557	-	360	WP_000581200.1	holo-ACP synthase	-
B9T45_RS10815	2078561..2079052	-	492	WP_001205910.1	PH domain-containing protein	-
B9T45_RS10820	2079039..2080622	-	1584	WP_001294626.1	PH domain-containing protein	-
B9T45_RS10825	2080615..2081094	-	480	WP_001287088.1	hypothetical protein	-
B9T45_RS10830	2081302..2081862	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T75809 WP_000621175.1 NZ_CP020959:c2076732-2076370 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T75809 NZ_CP020959:c2076732-2076370 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT75809 WP_000948331.1 NZ_CP020959:c2076899-2076729 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT75809 NZ_CP020959:c2076899-2076729 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1