Detailed information of TA system TA075777

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2150063..2150592
• Replicon: chromosome
• Accession: NZ_CP020956
• Organism: Staphylococcus aureus strain C8879

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B9T41_RS11270
• Protein ID: WP_000621175.1
• Coordinates: 2150063..2150425 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: B9T41_RS11275
• Protein ID: WP_000948331.1
• Coordinates: 2150422..2150592 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9T41_RS11245	2147041..2147811	-	771	WP_001041103.1	RNA polymerase sigma factor SigB	-
B9T41_RS11250	2147786..2148265	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
B9T41_RS11255	2148267..2148593	-	327	WP_001052491.1	anti-sigma factor antagonist	-
B9T41_RS11260	2148712..2149713	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
B9T41_RS11270	2150063..2150425	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9T41_RS11275	2150422..2150592	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
B9T41_RS11280	2150677..2151825	-	1149	WP_001281145.1	alanine racemase	-
B9T41_RS11285	2151891..2152250	-	360	WP_000581200.1	holo-ACP synthase	-
B9T41_RS11290	2152254..2152745	-	492	WP_001205910.1	PH domain-containing protein	-
B9T41_RS11295	2152732..2154315	-	1584	WP_001294626.1	PH domain-containing protein	-
B9T41_RS11300	2154308..2154787	-	480	WP_001287088.1	hypothetical protein	-
B9T41_RS11305	2154995..2155555	-	561	WP_001092411.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T75777 WP_000621175.1 NZ_CP020956:c2150425-2150063 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T75777 NZ_CP020956:c2150425-2150063 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT75777 WP_000948331.1 NZ_CP020956:c2150592-2150422 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT75777 NZ_CP020956:c2150592-2150422 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1