Detailed information of TA system TA075764

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/Tad-HTH_37
• Location: 137353..138071
• Replicon: plasmid pRheCIAT894d
• Accession: NZ_CP020951
• Organism: Rhizobium sp. CIAT894

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: RHEC894_RS26970
• Protein ID: WP_085739798.1
• Coordinates: 137688..138071 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: RHEC894_RS26965
• Protein ID: WP_085739797.1
• Coordinates: 137353..137691 (-)
• Length: 113 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
RHEC894_RS26940	132833..133537	+	705	WP_085739793.1	ABC transporter permease subunit	-
RHEC894_RS26945	133565..134341	+	777	WP_085739794.1	ATP-binding cassette domain-containing protein	-
RHEC894_RS26950	134338..135831	+	1494	WP_085739795.1	histidine ammonia-lyase	-
RHEC894_RS26955	135845..136387	-	543	WP_085740126.1	hypothetical protein	-
RHEC894_RS26960	136579..137316	+	738	WP_085739796.1	phosphatase PAP2 family protein	-
RHEC894_RS26965	137353..137691	-	339	WP_085739797.1	helix-turn-helix domain-containing protein	Antitoxin
RHEC894_RS26970	137688..138071	-	384	WP_085739798.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
RHEC894_RS26975	138265..140826	-	2562	WP_085739799.1	FAD-dependent oxidoreductase	-
RHEC894_RS32740	140929..141243	-	315	WP_125461004.1	hypothetical protein	-
RHEC894_RS26980	141134..141373	-	240	WP_085739800.1	hypothetical protein	-
RHEC894_RS26985	141383..141643	-	261	WP_010066184.1	hypothetical protein	-
RHEC894_RS26990	141644..141964	-	321	WP_085740127.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..560456	560456

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (20-110)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-90)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14785.91 Da
• Isoelectric Point: 10.0455

>T75764 WP_085739798.1 NZ_CP020951:c138071-137688 [Rhizobium sp. CIAT894]
MENLPRPTRPLLWIASSRKDYAEFPPKVQEAFGFELFLAQTGQHPPSAKMLKGLGNGIVELVEDHDSDTYRAVYTVRFDR
AVYVLHCFKKKSKRGIRTPQSDIELIKHRFRDAEAHYAAKQNEERKR

Nucleotide

• Length: 384 bp

>T75764 NZ_CP020951:c138071-137688 [Rhizobium sp. CIAT894]
ATGGAAAATCTGCCCCGCCCTACCCGACCGCTCCTTTGGATAGCGTCGAGCCGCAAAGATTATGCGGAGTTTCCGCCCAA
AGTGCAGGAAGCTTTTGGCTTTGAGCTGTTTCTGGCACAGACGGGGCAACACCCTCCATCGGCAAAGATGCTCAAGGGAC
TCGGCAATGGTATAGTGGAGCTGGTCGAAGACCATGACAGCGATACCTACCGCGCCGTTTATACCGTCCGTTTTGATAGA
GCCGTTTATGTGCTGCACTGCTTCAAGAAGAAGTCCAAGCGCGGCATCAGAACCCCTCAGAGCGATATCGAGCTGATCAA
GCACCGTTTCCGCGACGCGGAAGCCCATTATGCAGCCAAACAAAATGAGGAGCGCAAGCGATGA

Antitoxin

Protein

• Length: 113 a.a.
• Molecular weight: 12580.55 Da
• Isoelectric Point: 8.7528

>AT75764 WP_085739797.1 NZ_CP020951:c137691-137353 [Rhizobium sp. CIAT894]
MKTEHVEVHAGHPNIFAELGLPDAENHFLKAQIVAALHRLVKERKLNQAKAGALMGITQPEVSRLFRGHFREYSVERLMS
FLTAFNQDVEIVVRPHAKAGETGHITFNAVMI

Nucleotide

• Length: 339 bp

>AT75764 NZ_CP020951:c137691-137353 [Rhizobium sp. CIAT894]
ATGAAAACCGAACATGTCGAGGTTCATGCCGGCCACCCCAACATCTTTGCGGAGCTGGGGCTACCGGACGCCGAAAACCA
CTTCTTGAAAGCGCAGATTGTGGCGGCCCTGCACCGCCTCGTCAAAGAGCGCAAACTCAACCAGGCGAAGGCCGGAGCCC
TTATGGGGATCACTCAACCGGAAGTGTCCCGCCTCTTCCGGGGGCATTTCCGGGAATACTCCGTTGAACGCCTCATGAGC
TTCCTGACAGCTTTCAACCAGGACGTTGAAATTGTCGTAAGACCTCATGCCAAGGCCGGAGAAACCGGCCACATAACCTT
CAACGCCGTCATGATTTAA