Detailed information of TA system TA075756

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 137677..138584
• Replicon: plasmid unamed1
• Accession: NZ_CP020944
• Organism: Pantoea ananatis strain PNA 97-1R

Toxin (Protein)

• Gene name: -
• Uniprot ID: D4GET9
• Locus tag: B9Q16_RS22160
• Protein ID: WP_013027861.1
• Coordinates: 137677..138144 (-)
• Length: 156 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A8A4JZ23
• Locus tag: B9Q16_RS22165
• Protein ID: WP_014598335.1
• Coordinates: 138141..138584 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9Q16_RS22140	133063..133296	-	234	WP_013027857.1	DUF333 domain-containing protein	-
B9Q16_RS22145	133384..133956	+	573	WP_014598338.1	NAD(P)H-dependent oxidoreductase	-
B9Q16_RS22150	134018..134953	-	936	WP_013027859.1	hypothetical protein	-
B9Q16_RS22155	135638..137032	-	1395	WP_014604629.1	flagellar filament capping protein FliD	-
B9Q16_RS22160	137677..138144	-	468	WP_013027861.1	RES family NAD+ phosphorylase	Toxin
B9Q16_RS22165	138141..138584	-	444	WP_014598335.1	DUF2384 domain-containing protein	Antitoxin
B9Q16_RS22170	138970..139194	+	225	WP_013027863.1	KTSC domain-containing protein	-
B9Q16_RS22175	139348..140043	+	696	WP_104933799.1	hypothetical protein	-
B9Q16_RS22180	140118..140807	-	690	WP_014598333.1	HTH-type transcriptional regulator RutR	-
B9Q16_RS22185	141206..142297	+	1092	WP_013027866.1	pyrimidine utilization protein A	-
B9Q16_RS22190	142294..143046	+	753	WP_019105823.1	pyrimidine utilization protein B	-
B9Q16_RS22195	143072..143458	+	387	WP_013027868.1	pyrimidine utilization protein C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	fliC / fliC	1..273809	273809

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (2-145)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (31-89)

Sequences

Toxin

Protein

• Length: 156 a.a.
• Molecular weight: 17207.70 Da
• Isoelectric Point: 5.1570

>T75756 WP_013027861.1 NZ_CP020944:c138144-137677 [Pantoea ananatis]
MKLYRMTKTRYLGSAWSGFGAREGGGRWNSVGVSMVYASETASLTMLETLIHLQSASVLDFFTLMSIDVPDRLIEWIDIK
QLPDDWAAPEAPAALRLFGDAWIQSGGSVALRVPSALSPVESNYLLNPEHPEFSAIVRQAVNIPFLFDARFSRQV

Nucleotide

• Length: 468 bp

>T75756 NZ_CP020944:c138144-137677 [Pantoea ananatis]
TTGAAGCTCTATCGCATGACCAAAACGCGCTATCTGGGGTCGGCCTGGAGCGGTTTTGGTGCCCGTGAAGGCGGCGGACG
CTGGAACAGCGTCGGCGTATCGATGGTCTACGCTTCTGAAACTGCTTCCCTCACCATGCTGGAGACGCTTATCCATCTGC
AAAGCGCCAGCGTGCTGGACTTTTTTACCCTCATGAGCATTGACGTTCCCGACAGGCTGATTGAATGGATTGATATCAAG
CAACTGCCTGACGACTGGGCCGCACCGGAAGCGCCTGCAGCGCTGCGTTTATTTGGTGATGCCTGGATACAAAGCGGGGG
ATCGGTCGCACTGCGCGTGCCCAGTGCCCTTTCGCCCGTGGAATCGAATTATCTTCTCAATCCCGAGCATCCTGAATTCA
GTGCGATCGTCAGACAGGCGGTAAACATCCCCTTCCTTTTTGACGCACGTTTTTCCCGACAGGTATAA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16375.69 Da
• Isoelectric Point: 7.0208

>AT75756 WP_014598335.1 NZ_CP020944:c138584-138141 [Pantoea ananatis]
MKQFTLPVSAAEPDRLWQFAGLSSADGVMLMDQIDDGLDGTVASRIVTWAQISQAELRRMTGIAGSTFTRGVKSRFTAEQ
SERLVRFIRVMDRAVELFEGDKEKARQWLNEPARALGNKKPADLVASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT75756 NZ_CP020944:c138584-138141 [Pantoea ananatis]
ATGAAACAGTTCACTTTACCCGTATCCGCTGCAGAACCGGATCGTCTATGGCAATTTGCTGGCCTGAGCAGTGCTGACGG
CGTGATGCTGATGGATCAAATCGATGATGGTCTGGACGGCACGGTAGCCAGTCGCATTGTCACCTGGGCGCAAATCTCTC
AGGCAGAACTGCGCCGTATGACCGGCATTGCGGGCTCAACCTTCACCCGCGGCGTTAAAAGCCGCTTTACTGCGGAGCAG
AGCGAACGGCTGGTGCGTTTTATTCGCGTGATGGATCGTGCGGTTGAGCTGTTTGAGGGTGATAAAGAAAAGGCACGGCA
GTGGCTCAATGAGCCTGCGCGCGCCTTAGGCAATAAAAAACCGGCCGATCTGGTTGCGTCAGAAACCGGTGCTTATGAAG
TGCTGAAACTCATTACCCGGCTTGAACACGGTGTGTATTCTTGA