Detailed information of TA system TA075744

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1924655..1925245
• Replicon: chromosome
• Accession: NZ_CP020943
• Organism: Pantoea ananatis strain PNA 97-1R

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: B9Q16_RS09265
• Protein ID: WP_029570198.1
• Coordinates: 1924913..1925245 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: B9Q16_RS09260
• Protein ID: WP_019106593.1
• Coordinates: 1924655..1924912 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9Q16_RS09225	1919946..1920371	+	426	WP_104933449.1	pentapeptide repeat-containing protein	-
B9Q16_RS09230	1920663..1921901	-	1239	WP_104933448.1	MFS transporter	-
B9Q16_RS09240	1922649..1923611	+	963	WP_104933446.1	ferrochelatase	-
B9Q16_RS09250	1924102..1924307	+	206	Protein_1771	helix-turn-helix domain-containing protein	-
B9Q16_RS09260	1924655..1924912	+	258	WP_019106593.1	hypothetical protein	Antitoxin
B9Q16_RS09265	1924913..1925245	+	333	WP_029570198.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B9Q16_RS09275	1925506..1926339	-	834	WP_013026205.1	DgsA anti-repressor MtfA	-
B9Q16_RS09280	1926445..1927122	-	678	WP_104933444.1	hypothetical protein	-
B9Q16_RS09285	1927734..1928438	+	705	WP_022623305.1	phosphohydrolase	-
B9Q16_RS09290	1928512..1928766	+	255	WP_013026202.1	Arc family DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-83)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11930.65 Da
• Isoelectric Point: 8.0219

>T75744 WP_029570198.1 NZ_CP020943:1924913-1925245 [Pantoea ananatis]
MDRGEIWLVSLDPNAGHEQSGRRPVLIVSPASFNQFTRLPVVVPVTNGGNFARAAGFTVSLNGAGTKTTGVIRCDQPRTL
DMEARNGKYLERIPEVIVNEVVARLEAILS

Nucleotide

• Length: 333 bp

>T75744 NZ_CP020943:1924913-1925245 [Pantoea ananatis]
ATGGACAGAGGGGAAATCTGGCTCGTTTCACTAGATCCGAATGCCGGGCATGAACAAAGCGGAAGGCGTCCAGTACTGAT
TGTTTCTCCGGCGTCCTTTAATCAGTTTACGCGGCTACCTGTGGTTGTTCCCGTGACCAACGGCGGAAACTTTGCCCGCG
CAGCGGGGTTTACTGTTTCACTGAATGGCGCAGGAACGAAAACAACGGGGGTAATTCGTTGTGACCAGCCCAGAACCCTT
GATATGGAAGCCCGGAACGGCAAATATCTTGAACGGATACCTGAAGTTATTGTGAATGAAGTGGTGGCCAGACTGGAGGC
CATTCTGAGCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9283.76 Da
• Isoelectric Point: 4.5821

>AT75744 WP_019106593.1 NZ_CP020943:1924655-1924912 [Pantoea ananatis]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDSGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPTV
GKELL

Nucleotide

• Length: 258 bp

>AT75744 NZ_CP020943:1924655-1924912 [Pantoea ananatis]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCTGTCATGCTGGCGGTCCCTCCCGCCGTGCTGAAAACGCTGGGGCT
GTCGACGGACAGCGAAGTGGGCATGACCATTGATAGTGGCTGCCTCATAATTGAACCTCAAAAACGTCCCCGCTATTCAC
TCGAAGAACTACTGGCGCAGTGCGATCCGCACGCTGAAATAAGCGAAGAGGGTCGGGAATGGATTGATGCGCCTACGGTG
GGCAAGGAGCTCCTGTAG