Detailed information of TA system TA075743

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pmenTA/darT(toxin)
• Location: 4938742..4939487
• Replicon: chromosome
• Accession: NZ_CP020942
• Organism: Xanthomonas oryzae pv. oryzae strain PXO61

Toxin (Protein)

• Gene name: pmenT
• Uniprot ID: -
• Locus tag: B9W05_RS24465
• Protein ID: WP_199285672.1
• Coordinates: 4939158..4939487 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: pmenA
• Uniprot ID: -
• Locus tag: B9W05_RS24460
• Protein ID: WP_011407754.1
• Coordinates: 4938742..4939161 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W05_RS24435	4935030..4935356	-	327	Protein_4425	hypothetical protein	-
B9W05_RS24440 (B9W05_24045)	4935658..4936893	+	1236	WP_011257570.1	ISL3-like element ISXoo13 family transposase	-
B9W05_RS24445 (B9W05_24050)	4936931..4937611	-	681	WP_011257926.1	phosphate phosphatase	-
B9W05_RS24450 (B9W05_24055)	4937722..4938690	-	969	WP_011407317.1	IS5-like element ISXo1 family transposase	-
B9W05_RS24460 (B9W05_24060)	4938742..4939161	-	420	WP_011407754.1	macro domain-containing protein	Antitoxin
B9W05_RS24465 (B9W05_24065)	4939158..4939487	-	330	WP_199285672.1	DUF4433 domain-containing protein	Toxin
B9W05_RS24470 (B9W05_24070)	4939590..4940909	+	1320	WP_109182095.1	IS701 family transposase	-
B9W05_RS24475 (B9W05_24075)	4940953..4941288	-	336	WP_011407757.1	DarT ssDNA thymidine ADP-ribosyltransferase family protein	-
B9W05_RS24480 (B9W05_24080)	4941620..4942546	-	927	WP_011257932.1	Grx4 family monothiol glutaredoxin	-
B9W05_RS24485 (B9W05_24085)	4942592..4943383	+	792	WP_011407758.1	polysaccharide deacetylase family protein	-
B9W05_RS24495 (B9W05_24095)	4943685..4944356	-	672	WP_011407759.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	4935658..4940909	5251

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• ssDNA thymidine ADP-ribosyltransferase, DarT (3-108)

Antitoxin

• Macro domain (18-130)

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 13145.86 Da
• Isoelectric Point: 7.1160

>T75743 WP_199285672.1 NZ_CP020942:c4939487-4939158 [Xanthomonas oryzae pv. oryzae]
MHHVAERRWPFLFTDSHAYCQLASFYSDLSDLDKIDWPLLQARDFRRDPDDPAKFERYQAEALIHRHLPLDGLRGIVCHT
DGLKQTIERQLQERNLKLPVHTRTDWYFR

Nucleotide

• Length: 330 bp

>T75743 NZ_CP020942:c4939487-4939158 [Xanthomonas oryzae pv. oryzae]
TTGCATCATGTCGCCGAACGCCGATGGCCGTTTCTGTTTACTGACAGCCACGCCTACTGCCAGTTGGCGAGCTTCTATTC
CGATTTATCTGATCTCGACAAGATCGACTGGCCGTTGCTCCAAGCACGCGATTTCCGGCGCGACCCAGACGACCCAGCCA
AGTTCGAGCGCTATCAGGCCGAGGCCCTGATTCACCGACACTTGCCGCTTGATGGCCTGCGCGGCATTGTGTGTCATACG
GATGGTTTGAAACAGACCATCGAGCGGCAACTCCAAGAGCGAAACCTGAAATTGCCGGTTCATACCCGCACCGATTGGTA
TTTCCGATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15607.02 Da
• Isoelectric Point: 6.4733

>AT75743 WP_011407754.1 NZ_CP020942:c4939161-4938742 [Xanthomonas oryzae pv. oryzae]
MITFTQGNLLESGAEALVNTVNTVGVMGKGIALMFKERFKENFLRYAAACKDNQVRIGKIFVTEVNELDGPRWIINFPTK
QHWRGDSRIEWITEGLQDLHRFLIENKVKSIAIPPLGAGNGGLDWAEVRPLIEEVLGNL

Nucleotide

• Length: 420 bp

>AT75743 NZ_CP020942:c4939161-4938742 [Xanthomonas oryzae pv. oryzae]
ATGATCACGTTTACACAAGGCAATCTACTGGAGTCTGGCGCAGAGGCGCTGGTCAACACCGTCAACACCGTCGGCGTGAT
GGGCAAGGGCATCGCGCTGATGTTCAAGGAGCGCTTCAAGGAGAACTTCCTCCGATATGCCGCCGCCTGCAAAGACAACC
AAGTGCGCATTGGCAAAATTTTCGTCACCGAAGTCAACGAACTGGACGGGCCACGCTGGATCATCAATTTTCCGACCAAA
CAGCACTGGCGTGGCGATTCCCGCATCGAATGGATCACTGAAGGATTGCAGGACTTGCACCGTTTTCTGATCGAGAACAA
AGTCAAATCCATTGCCATCCCGCCACTGGGCGCGGGCAATGGAGGATTGGACTGGGCCGAGGTGCGCCCACTGATCGAAG
AAGTACTAGGGAACCTCTGA