Detailed information of TA system TA075728

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/-
• Location: 19577..19953
• Replicon: plasmid unnamed1
• Accession: NZ_CP020934
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: parE
• Uniprot ID: A0A075MDK3
• Locus tag: B9W17_RS23860
• Protein ID: WP_000969988.1
• Coordinates: 19577..19759 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: -
• Locus tag: B9W17_RS23865
• Protein ID: WP_001324224.1
• Coordinates: 19756..19953 (-)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS23845	17576..18610	-	1035	WP_001190234.1	3-deoxy-7-phosphoheptulonate synthase	-
B9W17_RS23855	19170..19478	-	309	WP_000377483.1	hypothetical protein	-
B9W17_RS23860	19577..19759	-	183	WP_000969988.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
B9W17_RS23865	19756..19953	-	198	WP_001324224.1	toxin-antitoxin system protein	Antitoxin
B9W17_RS23870	20635..21909	+	1275	WP_000489609.1	HlyD family secretion protein	-
B9W17_RS23875	21884..23998	+	2115	WP_001183604.1	peptidase domain-containing ABC transporter	-
B9W17_RS23880	24168..24479	-	312	WP_001259758.1	colicin V	-
B9W17_RS23885	24457..24693	-	237	WP_001323890.1	colicin V immunity protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	oqxA / oqxB / sul2 / aph(3'')-Ib / aph(6)-Id / aph(3')-Ia / sitABCD	iroB / iroC / iroD / iroE / iroN / iutA / iucD / iucC / iucB / iucA	1..151137	151137

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 6931.07 Da
• Isoelectric Point: 4.5349

>T75728 WP_000969988.1 NZ_CP020934:c19759-19577 [Escherichia coli]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGCSASLLFSLSFISVQVHDNIITV

Nucleotide

• Length: 183 bp

>T75728 NZ_CP020934:c19759-19577 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
AGCCGCCATTGAACTGGACGAACGGATTGGTTGTTCAGCCAGCCTCCTGTTCTCCCTTTCTTTTATCAGTGTACAGGTGC
ATGACAACATAATCACGGTATGA

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 7658.53 Da
• Isoelectric Point: 7.2046

>AT75728 WP_001324224.1 NZ_CP020934:c19953-19756 [Escherichia coli]
MDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRDEMSRKVAGK

Nucleotide

• Length: 198 bp

>AT75728 NZ_CP020934:c19953-19756 [Escherichia coli]
ATGGACCGTAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTGGAACGGCAGCATAATACCTGGTTCCGTGA
CCAGGTTGAGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCAT
GGCGGGATGAAATGAGCAGGAAAGTCGCCGGTAAATGA

Structures

Toxin

• AlphaFold DB: A0A075MDK3