Detailed information of TA system TA075713

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1683591..1684426
• Replicon: chromosome
• Accession: NZ_CP020933
• Organism: Escherichia coli strain HB-Coli0

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A2S4A272
• Locus tag: B9W17_RS08280
• Protein ID: WP_001094443.1
• Coordinates: 1683591..1683968 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: B9W17_RS08285
• Protein ID: WP_085704244.1
• Coordinates: 1684058..1684426 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9W17_RS25335	1678971..1681224	-	2254	Protein_1562	alpha-amylase family protein	-
B9W17_RS08265	1681959..1682804	-	846	WP_001280427.1	DUF4942 domain-containing protein	-
B9W17_RS08270	1682889..1683086	-	198	WP_000445281.1	DUF957 domain-containing protein	-
B9W17_RS08275	1683106..1683594	-	489	WP_000761669.1	hypothetical protein	-
B9W17_RS08280	1683591..1683968	-	378	WP_001094443.1	TA system toxin CbtA family protein	Toxin
B9W17_RS08285	1684058..1684426	-	369	WP_085704244.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
B9W17_RS08290	1684500..1684721	-	222	WP_000692176.1	DUF987 domain-containing protein	-
B9W17_RS08295	1684808..1685284	-	477	WP_001360063.1	RadC family protein	-
B9W17_RS08300	1685300..1685785	-	486	WP_000206656.1	antirestriction protein	-
B9W17_RS08305	1685877..1686695	-	819	WP_021520251.1	DUF945 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1679578..1705503	25925

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14058.97 Da
• Isoelectric Point: 7.3523

>T75713 WP_001094443.1 NZ_CP020933:c1683968-1683591 [Escherichia coli]
MNTLPDTHVREASHCQSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMARDNYRTVNNITLGKHPGAKQ

Nucleotide

• Length: 378 bp

>T75713 NZ_CP020933:c1683968-1683591 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACTCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13748.60 Da
• Isoelectric Point: 6.7393

>AT75713 WP_085704244.1 NZ_CP020933:c1684426-1684058 [Escherichia coli]
VSDTLPGTTHPDDNHDRLWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKRLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT75713 NZ_CP020933:c1684426-1684058 [Escherichia coli]
GTGTCAGACACACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCTCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A2S4A272