Detailed information of TA system TA075686

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3092929..3093565
• Replicon: chromosome
• Accession: NZ_CP020915
• Organism: Bacillus subtilis strain 50-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G4NU33
• Locus tag: B9L64_RS15855
• Protein ID: WP_003156187.1
• Coordinates: 3093215..3093565 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G4NU32
• Locus tag: B9L64_RS15850
• Protein ID: WP_003225183.1
• Coordinates: 3092929..3093210 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B9L64_RS15830	3089288..3089887	-	600	WP_032722928.1	rhomboid family intramembrane serine protease	-
B9L64_RS15835	3089982..3090347	+	366	WP_015252768.1	holo-ACP synthase	-
B9L64_RS15840	3090513..3091529	+	1017	WP_003234282.1	outer membrane lipoprotein carrier protein LolA	-
B9L64_RS15845	3091644..3092813	+	1170	WP_015252766.1	alanine racemase	-
B9L64_RS15850	3092929..3093210	+	282	WP_003225183.1	type II toxin-antitoxin system antitoxin EndoAI	Antitoxin
B9L64_RS15855	3093215..3093565	+	351	WP_003156187.1	type II toxin-antitoxin system endoribonuclease NdoA	Toxin
B9L64_RS15860	3093680..3094504	+	825	WP_009966610.1	RsbT co-antagonist protein RsbRA	-
B9L64_RS15865	3094509..3094874	+	366	WP_003225190.1	RsbT antagonist protein RsbS	-
B9L64_RS15870	3094878..3095279	+	402	WP_003246640.1	serine/threonine-protein kinase RsbT	-
B9L64_RS15875	3095291..3096298	+	1008	WP_003234295.1	phosphoserine phosphatase RsbU	-
B9L64_RS15880	3096360..3096689	+	330	WP_003234298.1	anti-sigma factor antagonist RsbV	-
B9L64_RS15885	3096686..3097168	+	483	WP_003234299.1	anti-sigma B factor RsbW	-
B9L64_RS15890	3097134..3097922	+	789	WP_003246715.1	RNA polymerase sigma factor SigB	-
B9L64_RS15895	3097922..3098521	+	600	WP_003246608.1	phosphoserine phosphatase RsbX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (12-45)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12977.98 Da
• Isoelectric Point: 4.8781

>T75686 WP_003156187.1 NZ_CP020915:3093215-3093565 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Nucleotide

• Length: 351 bp

>T75686 NZ_CP020915:3093215-3093565 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10546.92 Da
• Isoelectric Point: 6.4708

>AT75686 WP_003225183.1 NZ_CP020915:3092929-3093210 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Nucleotide

• Length: 282 bp

>AT75686 NZ_CP020915:3092929-3093210 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Structures

Toxin

• PDB: 4MDX
• PDB: 4ME7
• PDB: 1NE8

Antitoxin

• AlphaFold DB: G4NU32