Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3092929..3093565 Replicon chromosome
Accession NZ_CP020915
Organism Bacillus subtilis strain 50-1

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag B9L64_RS15855 Protein ID WP_003156187.1
Coordinates 3093215..3093565 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G4NU32
Locus tag B9L64_RS15850 Protein ID WP_003225183.1
Coordinates 3092929..3093210 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B9L64_RS15830 3089288..3089887 - 600 WP_032722928.1 rhomboid family intramembrane serine protease -
B9L64_RS15835 3089982..3090347 + 366 WP_015252768.1 holo-ACP synthase -
B9L64_RS15840 3090513..3091529 + 1017 WP_003234282.1 outer membrane lipoprotein carrier protein LolA -
B9L64_RS15845 3091644..3092813 + 1170 WP_015252766.1 alanine racemase -
B9L64_RS15850 3092929..3093210 + 282 WP_003225183.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
B9L64_RS15855 3093215..3093565 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
B9L64_RS15860 3093680..3094504 + 825 WP_009966610.1 RsbT co-antagonist protein RsbRA -
B9L64_RS15865 3094509..3094874 + 366 WP_003225190.1 RsbT antagonist protein RsbS -
B9L64_RS15870 3094878..3095279 + 402 WP_003246640.1 serine/threonine-protein kinase RsbT -
B9L64_RS15875 3095291..3096298 + 1008 WP_003234295.1 phosphoserine phosphatase RsbU -
B9L64_RS15880 3096360..3096689 + 330 WP_003234298.1 anti-sigma factor antagonist RsbV -
B9L64_RS15885 3096686..3097168 + 483 WP_003234299.1 anti-sigma B factor RsbW -
B9L64_RS15890 3097134..3097922 + 789 WP_003246715.1 RNA polymerase sigma factor SigB -
B9L64_RS15895 3097922..3098521 + 600 WP_003246608.1 phosphoserine phosphatase RsbX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T75686 WP_003156187.1 NZ_CP020915:3093215-3093565 [Bacillus subtilis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T75686 NZ_CP020915:3093215-3093565 [Bacillus subtilis]
TTGATTGTGAAACGCGGCGATGTTTATTTTGCTGATTTATCTCCTGTTGTTGGCTCAGAGCAAGGCGGGGTGCGCCCGGT
TTTAGTGATCCAAAATGACATCGGAAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCACAAATACAGAAAG
CGAAATTACCAACCCACGTCGAAATCGATGCAAAACGCTACGGTTTTGAAAGAGATTCCGTTATTTTGCTGGAGCAAATT
CGGACGATTGACAAGCAAAGGTTAACGGATAAGATTACTCATCTGGATGATGAAATGATGGATAAGGTTGATGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10546.92 Da        Isoelectric Point: 6.4708

>AT75686 WP_003225183.1 NZ_CP020915:3092929-3093210 [Bacillus subtilis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHFAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT75686 NZ_CP020915:3092929-3093210 [Bacillus subtilis]
TTGTCTGAATCCAGCGCAAGAACCGAAATGAAAATCAGCTTGCCCGAAAACCTAGTAGCTGAATTGGATGGTGTAGCGAT
GCGGGAGAAACGAAGCAGAAACGAACTGATATCACAAGCAGTGAGAGCGTATGTCAGCGAACGAACAACTCGTCACAACC
GTGATTTGATGAGACGCGGCTATATGGAAATGGCGAAAATCAACCTGAATATTTCTTCTGAGGCTCACTTTGCGGAGTGC
GAGGCTGAAACGACAGTTGAGCGCTTAGTCAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
PDB 4ME7
AlphaFold DB G4NU32

References