Detailed information of TA system TA075675

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2904..3466
• Replicon: plasmid pK66-45-4
• Accession: NZ_CP020905
• Organism: Klebsiella pneumoniae strain K66-45

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: B5G58_RS30885
• Protein ID: WP_001623607.1
• Coordinates: 2904..3221 (-)
• Length: 106 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: -
• Locus tag: B5G58_RS30890
• Protein ID: WP_060544567.1
• Coordinates: 3221..3466 (-)
• Length: 82 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5G58_RS30865	1079..1537	+	459	WP_060544570.1	hypothetical protein	-
B5G58_RS30870	1729..2034	-	306	WP_060544569.1	hypothetical protein	-
B5G58_RS30875	2373..2594	-	222	WP_060544593.1	hypothetical protein	-
B5G58_RS30880	2619..2831	-	213	Protein_4	hypothetical protein	-
B5G58_RS30885	2904..3221	-	318	WP_001623607.1	CcdB family protein	Toxin
B5G58_RS30890	3221..3466	-	246	WP_060544567.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
B5G58_RS32105	3767..3940	-	174	WP_001610337.1	hypothetical protein	-
B5G58_RS30900	3988..4176	-	189	WP_001610336.1	hypothetical protein	-
B5G58_RS30905	4953..5456	-	504	WP_060544658.1	hypothetical protein	-
B5G58_RS30910	5440..5736	-	297	WP_001610345.1	hypothetical protein	-
B5G58_RS30915	5803..6366	-	564	WP_001610343.1	hypothetical protein	-
B5G58_RS30920	6416..6940	-	525	WP_001610342.1	DnaJ domain-containing protein	-
B5G58_RS30930	7344..7673	-	330	WP_001610340.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..41111	41111

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-104)

Antitoxin

• Post-segregation antitoxin CcdA (9-75)

Sequences

Toxin

Protein

• Length: 106 a.a.
• Molecular weight: 11782.77 Da
• Isoelectric Point: 8.4499

>T75675 WP_001623607.1 NZ_CP020905:c3221-2904 [Klebsiella pneumoniae]
VKQFAVYKNKSRNKQAYPYFIDVQADMLAHLNTRLVMPLTQKANSNSQVKALTPIITIDQIDYVVLATMITTTDVKNLKA
EDAVMDASHLRDQLVSAIDMMILGI

Nucleotide

• Length: 318 bp

>T75675 NZ_CP020905:c3221-2904 [Klebsiella pneumoniae]
GTGAAACAGTTTGCAGTTTACAAAAATAAATCCCGGAACAAACAGGCATACCCGTATTTTATTGATGTGCAGGCAGATAT
GCTTGCACACCTTAATACTCGCCTAGTTATGCCTTTAACGCAGAAAGCTAATTCTAATTCGCAGGTAAAAGCGTTAACGC
CAATTATTACAATCGATCAGATCGATTATGTAGTGCTGGCAACCATGATAACGACAACGGATGTAAAAAATCTGAAAGCT
GAAGATGCAGTTATGGATGCTTCACATTTACGCGATCAATTGGTTTCTGCGATTGATATGATGATTTTAGGTATTTAA

Antitoxin

Protein

• Length: 82 a.a.
• Molecular weight: 9212.28 Da
• Isoelectric Point: 4.4243

>AT75675 WP_060544567.1 NZ_CP020905:c3466-3221 [Klebsiella pneumoniae]
MRTNVAEKKRSTNVYLNAGLLEEAKELDLNISAISNQALETAVRERKRERWIEENRAGIEALNSFVEEVGIFSDDENFGV
I

Nucleotide

• Length: 246 bp

>AT75675 NZ_CP020905:c3466-3221 [Klebsiella pneumoniae]
ATGAGAACGAATGTAGCCGAGAAAAAACGTAGTACAAACGTGTACTTAAATGCAGGTCTACTTGAAGAGGCTAAAGAGCT
GGACTTAAACATTTCCGCGATATCTAACCAGGCGCTGGAAACGGCTGTCAGAGAACGCAAGCGTGAACGCTGGATCGAGG
AAAACCGCGCGGGCATCGAGGCGTTAAACAGCTTCGTGGAAGAGGTGGGTATTTTTTCAGATGATGAAAATTTCGGGGTG
ATTTAG