Detailed information of TA system TA075674

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 142470..143215
• Replicon: plasmid pK66-45-2
• Accession: NZ_CP020903
• Organism: Klebsiella pneumoniae strain K66-45

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A331KSM6
• Locus tag: B5G58_RS29740
• Protein ID: WP_032408901.1
• Coordinates: 142470..142961 (-)
• Length: 164 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: B5G58_RS29745
• Protein ID: WP_014386183.1
• Coordinates: 142949..143215 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5G58_RS29715	138154..138909	+	756	WP_032408898.1	DeoR/GlpR family DNA-binding transcription regulator	-
B5G58_RS29720	139171..139611	+	441	WP_014386179.1	IS66 family insertion sequence hypothetical protein	-
B5G58_RS29725	139608..139958	+	351	WP_014386180.1	IS66 family insertion sequence element accessory protein TnpB	-
B5G58_RS29730	139989..141581	+	1593	Protein_154	IS66 family transposase	-
B5G58_RS29735	141778..142029	+	252	WP_032408900.1	aldo/keto reductase	-
B5G58_RS29740	142470..142961	-	492	WP_032408901.1	GNAT family N-acetyltransferase	Toxin
B5G58_RS29745	142949..143215	-	267	WP_014386183.1	DUF1778 domain-containing protein	Antitoxin
B5G58_RS29750	143412..143570	+	159	WP_014386184.1	type I toxin-antitoxin system Hok family toxin	-
B5G58_RS29755	143814..144299	-	486	WP_014386185.1	hypothetical protein	-
B5G58_RS32080	144489..144761	+	273	WP_032408902.1	hypothetical protein	-
B5G58_RS29765	144758..145108	+	351	WP_014386186.1	hypothetical protein	-
B5G58_RS29780	145745..146071	+	327	WP_014386187.1	hypothetical protein	-
B5G58_RS29785	146133..146345	+	213	WP_014386188.1	hypothetical protein	-
B5G58_RS29790	146356..146580	+	225	WP_014386189.1	hypothetical protein	-
B5G58_RS29795	146661..146981	+	321	WP_014386190.1	type II toxin-antitoxin system RelE/ParE family toxin	-
B5G58_RS29800	146971..147249	+	279	WP_004152721.1	helix-turn-helix domain-containing protein	-
B5G58_RS29805	147250..147663	+	414	WP_013023817.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia	-	1..200365	200365

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (89-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-87)

Sequences

Toxin

Protein

• Length: 164 a.a.
• Molecular weight: 17667.46 Da
• Isoelectric Point: 8.8757

>T75674 WP_032408901.1 NZ_CP020903:c142961-142470 [Klebsiella pneumoniae]
VGKVTAPVPLGTSHILAEFHCGEPVLDEWIKHRGLKNQSLGAARTFVVCRGNASQVLAYYSLATGSVTHAIAPGSLRRNM
PDPVPVIILARLAVDTSYHGQGLGADLLHDAVLRCCRVAENIGVRAVMVHALSDSARQFYIHHGFIPSQTQDRTLFLRLP
LIT

Nucleotide

• Length: 492 bp

>T75674 NZ_CP020903:c142961-142470 [Klebsiella pneumoniae]
GTGGGCAAAGTAACGGCTCCGGTCCCACTCGGTACGTCACACATCCTCGCTGAGTTTCACTGTGGTGAGCCTGTACTGGA
CGAATGGATAAAGCACCGGGGACTAAAAAATCAGTCCCTGGGGGCGGCCAGAACGTTTGTGGTGTGCCGGGGAAATGCTT
CACAGGTGCTTGCCTACTACTCTCTCGCGACCGGCAGCGTCACTCATGCCATTGCGCCGGGAAGCCTGCGAAGGAATATG
CCCGATCCTGTACCTGTCATCATCCTGGCCCGTCTTGCTGTCGATACGAGTTATCACGGCCAGGGACTGGGGGCTGATTT
ACTGCATGATGCCGTATTACGTTGCTGTCGGGTGGCTGAGAACATTGGCGTGCGGGCCGTTATGGTTCATGCACTCTCTG
ACAGTGCCAGACAGTTCTACATACATCACGGATTTATCCCTTCACAAACGCAGGATAGAACCCTTTTTTTACGGTTGCCT
CTCATTACTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10090.66 Da
• Isoelectric Point: 5.1169

>AT75674 WP_014386183.1 NZ_CP020903:c143215-142949 [Klebsiella pneumoniae]
MKSDVQLNIRAKEAQRALIDTAAEILHKSRTDFILEMACQAAENVILDRRMFNLNDEQYAEFIDMLDAPVTANPAMEKLL
ARKPQWAK

Nucleotide

• Length: 267 bp

>AT75674 NZ_CP020903:c143215-142949 [Klebsiella pneumoniae]
ATGAAATCTGATGTTCAGCTCAACATCCGGGCGAAAGAAGCCCAGCGGGCTCTGATTGATACTGCCGCTGAGATTCTCCA
CAAATCACGCACTGATTTTATACTGGAGATGGCCTGTCAGGCGGCGGAAAACGTCATTCTTGACCGCCGTATGTTCAATC
TCAATGATGAACAGTACGCCGAGTTTATCGACATGCTGGATGCGCCGGTCACCGCTAACCCGGCGATGGAGAAACTCCTG
GCGAGAAAACCGCAGTGGGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A331KSM6