Detailed information of TA system TA075673

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 118676..119319
• Replicon: plasmid pK66-45-2
• Accession: NZ_CP020903
• Organism: Klebsiella pneumoniae strain K66-45

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: B5G58_RS29600
• Protein ID: WP_014386165.1
• Coordinates: 118676..119092 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: -
• Locus tag: B5G58_RS29605
• Protein ID: WP_032408893.1
• Coordinates: 119089..119319 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5G58_RS29575	115296..115727	+	432	WP_014386161.1	conjugation system SOS inhibitor PsiB	-
B5G58_RS29580	115724..116452	+	729	WP_014386162.1	plasmid SOS inhibition protein A	-
B5G58_RS29585	116449..116775	+	327	WP_004152639.1	hypothetical protein	-
B5G58_RS29590	116831..117205	+	375	WP_032408891.1	hypothetical protein	-
B5G58_RS29595	117385..118353	-	969	WP_074422984.1	IS5 family transposase	-
B5G58_RS29600	118676..119092	-	417	WP_014386165.1	PIN domain-containing protein	Toxin
B5G58_RS29605	119089..119319	-	231	WP_032408893.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B5G58_RS29610	119639..120073	+	435	WP_000103648.1	RidA family protein	-
B5G58_RS29615	120078..120752	+	675	WP_014386166.1	DUF1028 domain-containing protein	-
B5G58_RS29620	120768..121925	+	1158	WP_032408895.1	acetylornithine deacetylase	-
B5G58_RS29625	122096..123244	+	1149	WP_014386168.1	ABC transporter ATP-binding protein	-
B5G58_RS29630	123251..124246	+	996	WP_000611945.1	NAD(P)H-quinone oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia	-	1..200365	200365
	-	flank	IS/Tn	-	-	117385..118308	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-130)

Antitoxin

• SpoVT-AbrB domain (7-55)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15127.44 Da
• Isoelectric Point: 7.8841

>T75673 WP_014386165.1 NZ_CP020903:c119092-118676 [Klebsiella pneumoniae]
MTKTYMLDTNICSYIMREQPEAVLKRLEQAVLRRHRIVVSAITYAEMRFGAIGKRASPRHVQLVDAFCARLDAILAWDRA
AVDATTEVKATLAAAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVAGLTFEDWVQ

Nucleotide

• Length: 417 bp

>T75673 NZ_CP020903:c119092-118676 [Klebsiella pneumoniae]
ATGACCAAAACCTACATGCTGGATACTAATATCTGCTCTTATATCATGCGCGAACAACCGGAAGCGGTGCTGAAACGCCT
AGAGCAAGCGGTGTTGCGCCGTCACCGGATAGTGGTGTCAGCCATCACCTATGCCGAAATGCGTTTTGGTGCCATCGGTA
AAAGGGCTTCGCCCCGTCATGTGCAGCTGGTGGACGCATTCTGTGCGCGCCTTGATGCCATACTGGCTTGGGATCGGGCC
GCAGTTGACGCTACAACAGAGGTTAAGGCCACGCTAGCAGCTGCCGGCACGCCAATCGGCCCAAACGACACAGCCATTGC
TGGACATGCCATTGCCGCTGGCGCCATCCTGGTGACGAATAATACGCGTGAATTTGAGCGAGTCGCTGGTCTGACGTTTG
AAGACTGGGTGCAATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8854.89 Da
• Isoelectric Point: 4.5112

>AT75673 WP_032408893.1 NZ_CP020903:c119319-119089 [Klebsiella pneumoniae]
MRTVSVFRNGNNRAIRLPRDLDFDGVNELEIIREGDTLILRPVKPSWSSFRQEEKADADFMAERENVVSDEGRFEL

Nucleotide

• Length: 231 bp

>AT75673 NZ_CP020903:c119319-119089 [Klebsiella pneumoniae]
ATGCGAACTGTATCTGTATTCAGGAACGGTAATAACCGGGCCATCCGTCTGCCCCGCGATCTGGACTTTGACGGCGTGAA
CGAACTGGAAATCATCCGCGAGGGCGATACCCTCATCCTGCGGCCGGTAAAACCCAGTTGGAGTTCGTTCCGCCAGGAGG
AAAAAGCTGACGCAGATTTTATGGCGGAGCGTGAAAATGTTGTTAGTGACGAGGGACGTTTTGAGCTATGA