Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 44114..44850 Replicon plasmid pK66-45-2
Accession NZ_CP020903
Organism Klebsiella pneumoniae strain K66-45

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag B5G58_RS29165 Protein ID WP_003026803.1
Coordinates 44114..44596 (-) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag B5G58_RS29170 Protein ID WP_003026799.1
Coordinates 44584..44850 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5G58_RS29150 40273..41619 - 1347 WP_020314316.1 ISNCY family transposase -
B5G58_RS29155 41849..42481 + 633 WP_001567369.1 hypothetical protein -
B5G58_RS29160 42510..43913 - 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
B5G58_RS29165 44114..44596 - 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
B5G58_RS29170 44584..44850 - 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
B5G58_RS29175 45026..45280 - 255 WP_004152108.1 hypothetical protein -
B5G58_RS29180 45356..45613 - 258 WP_004152107.1 hypothetical protein -
B5G58_RS29185 45662..45865 - 204 WP_004152106.1 hemolysin expression modulator Hha -
B5G58_RS29190 45899..46267 - 369 WP_004152105.1 hypothetical protein -
B5G58_RS29195 46311..46805 - 495 WP_004152104.1 DNA-binding protein -
B5G58_RS29200 46836..47411 - 576 WP_004152103.1 hypothetical protein -
B5G58_RS29205 47399..47668 - 270 WP_004152102.1 hypothetical protein -
B5G58_RS29210 48026..48376 + 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
B5G58_RS29215 48426..48788 + 363 WP_004152100.1 arsenite efflux transporter metallochaperone ArsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid aac(6')-Ib-cr / blaOXA-1 / tet(A) / aph(3')-Ia - 1..200365 200365


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T75672 WP_003026803.1 NZ_CP020903:c44596-44114 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T75672 NZ_CP020903:c44596-44114 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT75672 WP_003026799.1 NZ_CP020903:c44850-44584 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT75672 NZ_CP020903:c44850-44584 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References