Detailed information of TA system TA075671

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/ElaA-DUF1778
• Location: 222935..223686
• Replicon: plasmid pK66-45-1
• Accession: NZ_CP020902
• Organism: Klebsiella pneumoniae strain K66-45

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: H6U1U8
• Locus tag: B5G58_RS28210
• Protein ID: WP_014386536.1
• Coordinates: 223204..223686 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A071LPN3
• Locus tag: B5G58_RS28205
• Protein ID: WP_004902250.1
• Coordinates: 222935..223213 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5G58_RS31390	218563..218988	-	426	WP_000522996.1	organomercurial transporter MerC	-
B5G58_RS31395	219016..219291	-	276	WP_000732275.1	mercury resistance system periplasmic binding protein MerP	-
B5G58_RS28170	219307..219687	-	381	WP_001294654.1	mercuric ion transporter MerT	-
B5G58_RS28175	219759..220214	+	456	WP_001166628.1	Hg(II)-responsive transcriptional regulator	-
B5G58_RS28180	220867..221325	+	459	WP_014386535.1	hypothetical protein	-
B5G58_RS28195	222155..222496	+	342	WP_004902257.1	hypothetical protein	-
B5G58_RS28200	222604..222816	+	213	WP_004902255.1	hypothetical protein	-
B5G58_RS28205	222935..223213	+	279	WP_004902250.1	DUF1778 domain-containing protein	Antitoxin
B5G58_RS28210	223204..223686	+	483	WP_014386536.1	GNAT family N-acetyltransferase	Toxin
B5G58_RS28225	224721..225260	+	540	WP_004902239.1	hypothetical protein	-
B5G58_RS28230	225365..225757	+	393	WP_032442757.1	hypothetical protein	-
B5G58_RS28235	225858..226613	+	756	WP_004902235.1	DUF2971 domain-containing protein	-
B5G58_RS28240	226640..227287	+	648	WP_014386537.1	EcsC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / qnrS1 / aph(3')-VI / blaNDM-1 / blaCTX-M-15	-	1..338512	338512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (42-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (10-86)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17662.45 Da
• Isoelectric Point: 8.9130

>T75671 WP_014386536.1 NZ_CP020902:223204-223686 [Klebsiella pneumoniae]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T75671 NZ_CP020902:223204-223686 [Klebsiella pneumoniae]
ATGGGAATGAGAGCGCCTGAATCCCTGACGGCTGAACATAACATCGCTGAGTTCTGCTGCCAGGAGCCTGCCCTTAATGA
GTGGTTAAAGAAGAAGGCTCTCAGAAACCACAGTACCGGCATTTCCCGTGTCTATGTCGTCTGCGCAGAAAACACTAACC
GGGTCATCGGTTATTATTGTTTATCTTCGGGTAGCGTCCACCGTAACACAGTGCCTGGGGCCTATAGACGCAACGCGCCT
GATGTCATTCCAGTCATTGTTTTGGGACGGCTTGCCATCGACCAGGCCTGGGCCGGGAATGGGCTTGGTGCCGCACTTCT
TAAGGATGCTATATACCGTACGCAAAACATAGCCTTTCAGGTCGGGGTGAGAGCACTGGCCGTGCATGCGCTGAATGAAG
AAGTTAAATGTTTTTACACCCGTTTTGGCTTTGAGCCTTCCATAGTCAACACGCTGACATTGTTATTCCCGATAAAAGTC
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10704.23 Da
• Isoelectric Point: 4.5271

>AT75671 WP_004902250.1 NZ_CP020902:222935-223213 [Klebsiella pneumoniae]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT75671 NZ_CP020902:222935-223213 [Klebsiella pneumoniae]
ATGAAACCAGACGTTCGTGAAGCTCCGATTAATATCCGGGCCAAAACATCACAGCGTGACCTGATCGACATGGCCGCAAG
CCTGGTCTCAAAATCACGGACTGATTTTATGCTTGAGGTTGCCTGCAGGGAGGCACAGGACATTCTGCTTGATCAACGCC
TGTTCCTCCTCAATGACAATCAGTTTGAGGCGTTTATTGAAGAGCTTGACGCCCCAATAACGCCGGAGCGCCAGGCGCGA
ATCGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Toxin

• AlphaFold DB: A0A2A5MBI1

Antitoxin

• AlphaFold DB: A0A071LPN3