Detailed information of TA system TA075670

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 194852..195495
• Replicon: plasmid pK66-45-1
• Accession: NZ_CP020902
• Organism: Klebsiella pneumoniae strain K66-45

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: B5G58_RS28015
• Protein ID: WP_001044770.1
• Coordinates: 195079..195495 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: B5G58_RS28010
• Protein ID: WP_001261282.1
• Coordinates: 194852..195082 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B5G58_RS27975	189862..190131	+	270	WP_000339857.1	hypothetical protein	-
B5G58_RS27980	190488..191354	-	867	WP_004118283.1	replication initiation protein	-
B5G58_RS32040	191889..191993	+	105	WP_032409716.1	hypothetical protein	-
B5G58_RS27985	192122..192379	+	258	WP_000764642.1	hypothetical protein	-
B5G58_RS27990	192437..193213	-	777	WP_000015958.1	site-specific integrase	-
B5G58_RS27995	193210..193953	-	744	WP_000129823.1	hypothetical protein	-
B5G58_RS28000	194217..194423	-	207	Protein_203	hypothetical protein	-
B5G58_RS32045	194436..194895	-	460	Protein_204	hypothetical protein	-
B5G58_RS28010	194852..195082	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
B5G58_RS28015	195079..195495	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
B5G58_RS28020	195569..196279	+	711	WP_004152334.1	ATP-binding protein	-
B5G58_RS28025	196313..197010	+	698	Protein_208	IS1 family transposase	-
B5G58_RS28035	197323..198198	+	876	WP_000239590.1	class A extended-spectrum beta-lactamase CTX-M-15	-
B5G58_RS28040	198245..198577	-	333	WP_001393253.1	WbuC family cupin fold metalloprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / qnrS1 / aph(3')-VI / blaNDM-1 / blaCTX-M-15	-	1..338512	338512

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T75670 WP_001044770.1 NZ_CP020902:195079-195495 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T75670 NZ_CP020902:195079-195495 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT75670 WP_001261282.1 NZ_CP020902:194852-195082 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT75670 NZ_CP020902:194852-195082 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3