75669

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 70742..71264 Replicon plasmid pK66-45-1
Accession NZ_CP020902
Organism Klebsiella pneumoniae strain K66-45

Toxin (Protein)


Gene name stbE Uniprot ID A0A0C7KG00
Locus tag B5G58_RS27290 Protein ID WP_038991638.1
Coordinates 70742..71026 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag B5G58_RS27295 Protein ID WP_004181777.1
Coordinates 71016..71264 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B5G58_RS27265 65942..67037 + 1096 Protein_61 conjugal transfer protein TraG N-terminal domain-containing protein -
B5G58_RS27270 67136..68323 - 1188 WP_040120325.1 transposase -
B5G58_RS27275 68359..68787 + 429 WP_077256229.1 IS200/IS605 family transposase -
B5G58_RS27280 68866..69090 + 225 Protein_64 transposase -
B5G58_RS27285 69362..70588 - 1227 WP_040120327.1 transposase -
B5G58_RS31350 70624..70725 + 102 Protein_66 IS200/IS605 family transposase -
B5G58_RS27290 70742..71026 - 285 WP_038991638.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
B5G58_RS27295 71016..71264 - 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
B5G58_RS27300 71555..73354 + 1800 WP_038991636.1 ATP-dependent helicase -
B5G58_RS27305 73688..74674 + 987 WP_040120328.1 hypothetical protein -
B5G58_RS27310 74819..75172 + 354 WP_004181774.1 hypothetical protein -
B5G58_RS27315 75234..76055 + 822 WP_004181772.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid mph(E) / msr(E) / armA / sul1 / qacE / aadA2 / dfrA12 / qnrS1 / aph(3')-VI / blaNDM-1 / blaCTX-M-15 - 1..338512 338512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10993.73 Da        Isoelectric Point: 10.6516

>T75669 WP_038991638.1 NZ_CP020902:c71026-70742 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYHLTKHRN

Download         Length: 285 bp

>T75669 NZ_CP020902:c71026-70742 [Klebsiella pneumoniae]
ATGACTTATAAGCTGGCCTTTAATGAATCAGCTCTGAAAGAATGGAAAAAATTGGGCCATACCATTCGGGAACAATTCAA
AAAGAAGCTCGCTGAGCGCCTGTTGAACCCACGGGTGCCCGCATCGCAACTACATGGACGCAAAGACCAATACAAAATAA
AGCTCCGTGGCGCAGGCTACCGACTGGTTTACAGTGTCAACGATGATGTTGTGACGGTCACCGTAATTGGCGTCGGTAAA
CGTGAGAATGATGATATTTACCATTTAACCAAACACAGAAATTAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT75669 WP_004181777.1 NZ_CP020902:c71264-71016 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT75669 NZ_CP020902:c71264-71016 [Klebsiella pneumoniae]
ATGGCATTTCAGGTACTAACCACTACTGCAGCAAGCATCACAGAGCTTAAACGTGATCCTATGGGCACTTTCAATGCAGG
AGAAGGTGCCCCGGTAGCCATACTGAATCGCAACGAACCCGCTTTTTACTGTGTACCGCCTGCTCTGTATGCTCAACTGA
TGGAGCTTCTTGAAGATCAGGAGTTGGGTCGTATTGCTGATGAGCGAATTGGTGAGGCGGTAATCGAGGTCAGTCTAGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0C7KG00

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References