Detailed information of TA system TA075650

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 1024173..1024708
• Replicon: plasmid pRphaBra5d
• Accession: NZ_CP020900
• Organism: Rhizobium phaseoli Brasil 5 strain Bra5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Bra5_RS32085
• Protein ID: WP_085744662.1
• Coordinates: 1024433..1024708 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2AEZ7
• Locus tag: Bra5_RS32080
• Protein ID: WP_004677393.1
• Coordinates: 1024173..1024433 (+)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra5_RS32060	1020033..1020914	-	882	WP_012490008.1	ABC transporter permease	-
Bra5_RS32065	1020926..1021744	-	819	WP_085744660.1	ABC transporter ATP-binding protein	-
Bra5_RS32070	1021840..1022853	-	1014	WP_085744661.1	ABC transporter substrate-binding protein	-
Bra5_RS32075	1023144..1023872	-	729	WP_004677391.1	FadR family transcriptional regulator	-
Bra5_RS32080	1024173..1024433	+	261	WP_004677393.1	ribbon-helix-helix domain-containing protein	Antitoxin
Bra5_RS32085	1024433..1024708	+	276	WP_085744662.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Bra5_RS32090	1024715..1025722	-	1008	WP_010004849.1	LacI family transcriptional regulator	-
Bra5_RS32095	1025931..1027253	+	1323	WP_012490003.1	alpha-L-fucosidase	-
Bra5_RS32100	1027303..1028523	+	1221	WP_037145720.1	sugar ABC transporter substrate-binding protein	-
Bra5_RS32105	1028591..1029517	+	927	WP_029531794.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..1144005	1144005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (6-43)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10801.44 Da
• Isoelectric Point: 10.2283

>T75650 WP_085744662.1 NZ_CP020900:1024433-1024708 [Rhizobium phaseoli Brasil 5]
MEIKWTSKAVSDIARLYDFLSPVDRRAAARTVQALAAAPARLIEQPRLGERLDEFDPQKVRRILVGRYEMRYEIQQSTIY
VLRLWHTREDR

Nucleotide

• Length: 276 bp

>T75650 NZ_CP020900:1024433-1024708 [Rhizobium phaseoli Brasil 5]
ATGGAAATCAAGTGGACGAGCAAAGCTGTCTCCGACATCGCTCGCTTATACGACTTTCTCTCCCCGGTCGACCGGCGAGC
TGCGGCCCGAACCGTGCAGGCGCTGGCGGCAGCCCCCGCTCGTCTAATTGAGCAGCCACGTCTCGGCGAGCGGCTCGATG
AGTTCGATCCTCAGAAGGTCCGCCGCATTCTCGTTGGCCGTTATGAGATGCGTTATGAAATCCAGCAGTCGACAATCTAT
GTATTGCGGCTTTGGCATACGCGCGAGGATCGGTAG

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9686.93 Da
• Isoelectric Point: 4.6135

>AT75650 WP_004677393.1 NZ_CP020900:1024173-1024433 [Rhizobium phaseoli Brasil 5]
METKVLTAHVPLPLAQKVDQLASRLERSRGWIVKQALTAWIDQEEERRRLTLEALADVDSGNVVDHQSVQAWADSLDGDE
PISLLR

Nucleotide

• Length: 261 bp

>AT75650 NZ_CP020900:1024173-1024433 [Rhizobium phaseoli Brasil 5]
ATGGAGACCAAAGTTCTCACGGCTCATGTTCCATTGCCGCTTGCGCAGAAGGTCGATCAGCTTGCATCGCGCCTTGAGCG
TTCGCGCGGCTGGATCGTCAAGCAAGCCCTGACTGCATGGATCGACCAGGAAGAGGAGCGTCGGCGTTTGACGCTTGAGG
CGTTAGCCGATGTGGATAGCGGCAATGTTGTCGATCACCAGTCTGTTCAAGCCTGGGCCGATAGTCTTGACGGTGACGAG
CCGATTTCGTTGCTCCGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CQR5