Detailed information of TA system TA075649

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-RelB
• Location: 636427..637025
• Replicon: plasmid pRphaBra5d
• Accession: NZ_CP020900
• Organism: Rhizobium phaseoli Brasil 5 strain Bra5

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: Bra5_RS30170
• Protein ID: WP_080574712.1
• Coordinates: 636732..637025 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: F2A2S3
• Locus tag: Bra5_RS30165
• Protein ID: WP_004669386.1
• Coordinates: 636427..636735 (+)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra5_RS30140	632267..633013	+	747	WP_010004379.1	SDR family NAD(P)-dependent oxidoreductase	-
Bra5_RS30145	633257..633652	-	396	WP_041679408.1	helix-turn-helix transcriptional regulator	-
Bra5_RS30150	633789..634637	+	849	WP_085744500.1	SDR family oxidoreductase	-
Bra5_RS30155	634654..635037	+	384	WP_085744501.1	DoxX family protein	-
Bra5_RS30160	635071..636009	+	939	WP_085744502.1	NAD(P)-dependent alcohol dehydrogenase	-
Bra5_RS30165	636427..636735	+	309	WP_004669386.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
Bra5_RS30170	636732..637025	+	294	WP_080574712.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
Bra5_RS30175	637256..637627	+	372	WP_037144318.1	hypothetical protein	-
Bra5_RS30180	637663..638124	-	462	WP_085744503.1	DUF411 domain-containing protein	-
Bra5_RS30185	638180..638467	-	288	WP_085744504.1	copper-binding protein	-
Bra5_RS30190	638490..638969	-	480	WP_010005502.1	cupredoxin domain-containing protein	-
Bra5_RS30195	639004..640350	-	1347	WP_064844800.1	copper oxidase	-
Bra5_RS30200	640365..641831	-	1467	WP_085744505.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	gmd	1..1144005	1144005

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-90)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-53)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10596.14 Da
• Isoelectric Point: 5.7996

>T75649 WP_080574712.1 NZ_CP020900:636732-637025 [Rhizobium phaseoli Brasil 5]
VKLTWSAFALSDRDAIFTYIEAENPSAAILVDERIVAAVRRLIDFPASGRVGRIAGTRELVINGTPYVAAYAITETAVRI
LRVLHGAQEWPDTLPTS

Nucleotide

• Length: 294 bp

>T75649 NZ_CP020900:636732-637025 [Rhizobium phaseoli Brasil 5]
GTGAAGCTTACCTGGTCTGCCTTCGCATTATCGGATCGCGACGCCATATTCACATACATAGAAGCGGAAAATCCGTCAGC
CGCTATTCTGGTCGACGAACGGATCGTTGCTGCCGTTCGTCGGTTGATAGATTTTCCCGCGAGCGGTCGTGTCGGCAGAA
TTGCTGGGACCCGCGAACTGGTAATTAACGGTACGCCGTACGTCGCGGCGTATGCAATTACCGAAACAGCGGTCCGCATT
CTTCGCGTCCTCCATGGCGCGCAGGAATGGCCAGACACTTTGCCAACGAGCTAG

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11110.45 Da
• Isoelectric Point: 5.1751

>AT75649 WP_004669386.1 NZ_CP020900:636427-636735 [Rhizobium phaseoli Brasil 5]
MAANALVQTRIDAEVRDRASAVLENMGLTVSDAVRILLTRTANEGALPLELFSGSEAHDAWFRTKVLEALNDTRPDVSDD
EVEAQFAKRRAAARLNAGSRKS

Nucleotide

• Length: 309 bp

>AT75649 NZ_CP020900:636427-636735 [Rhizobium phaseoli Brasil 5]
ATGGCTGCAAATGCACTCGTGCAAACTCGTATTGATGCCGAAGTTCGGGATCGGGCCTCCGCAGTGCTTGAAAACATGGG
GCTTACAGTGTCGGACGCGGTTCGTATCTTGCTCACCCGGACGGCCAACGAGGGTGCGCTGCCACTCGAGCTTTTTTCAG
GCAGCGAGGCCCATGACGCTTGGTTTCGCACCAAGGTGCTTGAAGCGCTGAACGATACTCGACCGGACGTTTCGGATGAT
GAAGTCGAGGCACAATTTGCTAAACGTCGAGCGGCAGCCCGACTTAACGCGGGTTCTCGAAAATCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2U3CRA1