Detailed information of TA system TA075648

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 138441..139021
• Replicon: plasmid pRphaBra5c
• Accession: NZ_CP020899
• Organism: Rhizobium phaseoli Brasil 5 strain Bra5

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: Bra5_RS25670
• Protein ID: WP_085744016.1
• Coordinates: 138441..138824 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: Bra5_RS25675
• Protein ID: WP_064832110.1
• Coordinates: 138821..139021 (-)
• Length: 67 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra5_RS25655	134491..135690	+	1200	WP_085744015.1	mandelate racemase/muconate lactonizing enzyme family protein	-
Bra5_RS25660	135907..136206	-	300	WP_010001186.1	hypothetical protein	-
Bra5_RS25665	136401..138362	+	1962	WP_085744205.1	elongation factor G	-
Bra5_RS25670	138441..138824	-	384	WP_085744016.1	type II toxin-antitoxin system VapC family toxin	Toxin
Bra5_RS25675	138821..139021	-	201	WP_064832110.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
Bra5_RS25680	139360..141477	+	2118	WP_085744017.1	carboxy terminal-processing peptidase	-
Bra5_RS25685	141591..141773	+	183	WP_009999637.1	DUF1778 domain-containing protein	-
Bra5_RS25690	142025..142669	+	645	WP_125461169.1	hypothetical protein	-
Bra5_RS25695	142900..143796	+	897	WP_085744019.1	GGDEF domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..439779	439779

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-113)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 14251.33 Da
• Isoelectric Point: 7.1300

>T75648 WP_085744016.1 NZ_CP020899:c138824-138441 [Rhizobium phaseoli Brasil 5]
VILADTSIWIDHFRHADSELRRIIEDDRLLCHPAVIGELALGSLRDRNNVIAFLAAQRQAFVATHDEVMMMIDRHAVFSM
GIGYTDAHLIASVLLDRRAALWTRDKRLRAAAEKAGASLHTPDNARN

Nucleotide

• Length: 384 bp

>T75648 NZ_CP020899:c138824-138441 [Rhizobium phaseoli Brasil 5]
GTGATACTTGCCGACACGTCCATCTGGATCGATCATTTCCGGCATGCCGATTCAGAGCTGCGCAGGATTATCGAGGACGA
TCGTCTCTTATGCCATCCAGCCGTGATCGGAGAGCTCGCACTTGGCAGCCTTCGGGATCGCAATAACGTCATAGCTTTCT
TGGCGGCCCAGCGCCAAGCGTTCGTCGCGACGCACGACGAAGTCATGATGATGATCGATCGCCACGCCGTTTTCAGCATG
GGTATCGGCTACACAGATGCCCATTTGATCGCTTCGGTTCTACTCGATCGGCGAGCCGCCTTATGGACCAGAGACAAGCG
TCTGCGGGCAGCGGCCGAAAAGGCGGGGGCTTCACTGCACACGCCCGACAATGCGCGAAACTAA

Antitoxin

Protein

• Length: 67 a.a.
• Molecular weight: 7140.23 Da
• Isoelectric Point: 11.3760

>AT75648 WP_064832110.1 NZ_CP020899:c139021-138821 [Rhizobium phaseoli Brasil 5]
MRSTINLDDTLMERARSLTGTKETASLVRQALETLVRVESGKRLIALGGTMPDAEAAPRRRSAAAK

Nucleotide

• Length: 201 bp

>AT75648 NZ_CP020899:c139021-138821 [Rhizobium phaseoli Brasil 5]
ATGCGATCAACGATCAATCTCGACGACACCCTCATGGAAAGGGCGAGGTCCCTAACCGGCACGAAAGAAACCGCCTCTCT
GGTCCGCCAAGCGTTGGAGACGCTTGTTCGCGTGGAGTCGGGAAAACGCCTCATCGCGCTGGGCGGAACTATGCCGGATG
CGGAGGCAGCTCCGCGCCGCCGGAGCGCAGCCGCCAAGTGA