Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1693565..1694160 Replicon chromosome
Accession NZ_CP020896
Organism Rhizobium phaseoli Brasil 5 strain Bra5

Toxin (Protein)


Gene name vapC Uniprot ID A0A1W6GGE4
Locus tag Bra5_RS08375 Protein ID WP_064817946.1
Coordinates 1693565..1693954 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1W6GGF9
Locus tag Bra5_RS08380 Protein ID WP_064817947.1
Coordinates 1693951..1694160 (-) Length 70 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Bra5_RS08355 1688789..1689409 - 621 WP_085742253.1 glycerol-3-phosphate 1-O-acyltransferase PlsY -
Bra5_RS08360 1689455..1690744 - 1290 WP_064827372.1 dihydroorotase -
Bra5_RS08365 1690741..1691697 - 957 WP_004676639.1 aspartate carbamoyltransferase catalytic subunit -
Bra5_RS08370 1691876..1693534 + 1659 WP_010006241.1 acyl-CoA dehydrogenase family protein -
Bra5_RS08375 1693565..1693954 - 390 WP_064817946.1 PIN domain nuclease Toxin
Bra5_RS08380 1693951..1694160 - 210 WP_064817947.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
Bra5_RS08385 1694304..1697177 - 2874 WP_085742254.1 ribonuclease E/G -
Bra5_RS08390 1697800..1699074 + 1275 WP_085742255.1 N-acetylmuramoyl-L-alanine amidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-121)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(1-43)


Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 14778.09 Da        Isoelectric Point: 6.2388

>T75647 WP_064817946.1 NZ_CP020896:c1693954-1693565 [Rhizobium phaseoli Brasil 5]
VIVVDSSVWIAHLRGIESEARRRLQNIEDPLEILVGDVILLEVLQGARNERHAATIEASLRQFTLRPMLDDKLAVRAARN
YRLLRERGITVRKTIDLIIGTFCIQEGYSLLHDDRDFDPMAEYLGLRIA

Download         Length: 390 bp

>T75647 NZ_CP020896:c1693954-1693565 [Rhizobium phaseoli Brasil 5]
GTGATCGTCGTCGACAGTTCGGTCTGGATCGCGCATCTACGCGGGATAGAAAGTGAGGCCAGACGACGTCTGCAGAATAT
CGAGGATCCGCTCGAGATTCTTGTTGGTGACGTTATCCTTTTGGAAGTGCTTCAGGGGGCGCGCAACGAACGCCATGCAG
CCACCATCGAAGCGAGCTTGCGCCAATTCACTCTCCGCCCGATGCTTGACGACAAACTCGCTGTTAGGGCAGCTCGCAAC
TACCGTCTGTTACGGGAACGCGGCATCACAGTGCGCAAGACCATCGACCTGATCATCGGCACATTCTGCATTCAGGAAGG
CTATTCGCTCTTACACGATGATCGGGATTTCGATCCGATGGCCGAGTATCTCGGCCTCAGGATCGCCTGA

Antitoxin


Download         Length: 70 a.a.        Molecular weight: 7720.65 Da        Isoelectric Point: 5.7881

>AT75647 WP_064817947.1 NZ_CP020896:c1694160-1693951 [Rhizobium phaseoli Brasil 5]
MRTNIDIDDALLDAAMTATGLTTKKATIEQALRNLVDRHRRKNAIANLSGIGWEGDLDEMRRGRPDDNQ

Download         Length: 210 bp

>AT75647 NZ_CP020896:c1694160-1693951 [Rhizobium phaseoli Brasil 5]
ATGCGAACGAATATAGATATCGATGATGCGTTACTCGACGCAGCAATGACCGCCACCGGATTGACGACGAAAAAAGCCAC
CATCGAACAGGCCCTGCGAAACCTTGTCGACAGGCACCGCCGGAAAAACGCGATAGCAAATCTCTCAGGAATCGGCTGGG
AAGGGGATCTGGACGAGATGCGTCGCGGTCGTCCGGACGATAATCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1W6GGE4

Antitoxin

Source ID Structure
AlphaFold DB A0A1W6GGF9

References