Detailed information of TA system TA075646

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pasABC/RelE-HTH
• Location: 1073851..1074328
• Replicon: chromosome
• Accession: NZ_CP020896
• Organism: Rhizobium phaseoli Brasil 5 strain Bra5

Toxin (Protein)

• Gene name: pasB
• Uniprot ID: -
• Locus tag: Bra5_RS05265
• Protein ID: WP_085741946.1
• Coordinates: 1074059..1074328 (+)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: pasA
• Uniprot ID: F2A9C6
• Locus tag: Bra5_RS05260
• Protein ID: WP_004673983.1
• Coordinates: 1073851..1074075 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra5_RS05235	1069667..1070809	+	1143	WP_004673988.1	site-specific DNA-methyltransferase	-
Bra5_RS05240	1070908..1071273	-	366	WP_085741944.1	nuclear transport factor 2 family protein	-
Bra5_RS05245	1071278..1071892	-	615	WP_004673986.1	HAD family phosphatase	-
Bra5_RS05250	1071895..1072998	-	1104	WP_085741945.1	A/G-specific adenine glycosylase	-
Bra5_RS05255	1073095..1073592	+	498	WP_037115344.1	DUF721 domain-containing protein	-
Bra5_RS05260	1073851..1074075	+	225	WP_004673983.1	TraY domain-containing protein	Antitoxin
Bra5_RS05265	1074059..1074328	+	270	WP_085741946.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Bra5_RS05270	1074477..1075253	+	777	WP_085741947.1	DsbA family protein	-
Bra5_RS05275	1075359..1078820	+	3462	WP_085741948.1	chromosome segregation protein SMC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-88)

Antitoxin

• Relaxosome protein TraY (4-49)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10481.99 Da
• Isoelectric Point: 10.4070

>T75646 WP_085741946.1 NZ_CP020896:1074059-1074328 [Rhizobium phaseoli Brasil 5]
MAWSIEFHRAAERELEKLGHEAARRILRFLNDRVARLDDPRSIREALKGSELGDFWKYRVGDYRVIASIDDGAVRILVVR
IGNRRDVYR

Nucleotide

• Length: 270 bp

>T75646 NZ_CP020896:1074059-1074328 [Rhizobium phaseoli Brasil 5]
ATGGCGTGGAGCATTGAGTTTCACCGGGCAGCCGAGCGCGAGCTTGAGAAGCTTGGACATGAGGCGGCGCGCCGAATACT
GCGGTTTCTCAATGACCGCGTGGCGAGGCTGGACGATCCACGATCCATTCGAGAGGCGCTTAAAGGGTCTGAACTCGGGG
ATTTCTGGAAATATCGCGTTGGCGACTATCGAGTGATCGCAAGCATCGACGACGGGGCCGTTCGTATCCTCGTTGTGCGT
ATCGGCAACCGCCGTGACGTCTACCGATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8630.80 Da
• Isoelectric Point: 4.8894

>AT75646 WP_004673983.1 NZ_CP020896:1073851-1074075 [Rhizobium phaseoli Brasil 5]
MLALRLPPEIEARLDELSKRTGRSKSFYARQAILEHLDDLEDVYLAEKRLEELRRGESDTVSLAELMTRYGVEH

Nucleotide

• Length: 225 bp

>AT75646 NZ_CP020896:1073851-1074075 [Rhizobium phaseoli Brasil 5]
ATGTTGGCCTTGCGGTTGCCGCCTGAAATCGAAGCCCGGCTCGACGAGCTTTCCAAGCGTACGGGCCGCAGCAAGAGCTT
CTATGCCCGTCAGGCCATTCTCGAACATCTCGATGATCTTGAGGATGTCTATCTCGCCGAAAAGCGTCTTGAAGAACTTC
GGCGAGGTGAGAGTGATACCGTGTCGCTGGCCGAGCTGATGACGCGTTATGGCGTGGAGCATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1W6GER5