Detailed information of TA system TA075645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/CC2985(antitoxin)
• Location: 209086..209630
• Replicon: chromosome
• Accession: NZ_CP020896
• Organism: Rhizobium phaseoli Brasil 5 strain Bra5

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: Bra5_RS00985
• Protein ID: WP_085741536.1
• Coordinates: 209340..209630 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: F2A8A7
• Locus tag: Bra5_RS00980
• Protein ID: WP_004673546.1
• Coordinates: 209086..209337 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Bra5_RS00955	205087..206046	+	960	WP_085741534.1	glyoxylate/hydroxypyruvate reductase A	-
Bra5_RS00960	206242..206598	+	357	WP_010005829.1	hypothetical protein	-
Bra5_RS00965	206767..208071	+	1305	WP_004673542.1	nicotinate phosphoribosyltransferase	-
Bra5_RS00970	208068..208334	-	267	WP_085741535.1	hypothetical protein	-
Bra5_RS00975	208664..208873	-	210	WP_010023871.1	KTSC domain-containing protein	-
Bra5_RS00980	209086..209337	+	252	WP_004673546.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
Bra5_RS00985	209340..209630	+	291	WP_085741536.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
Bra5_RS00995	209978..210295	+	318	WP_004673550.1	hypothetical protein	-
Bra5_RS01000	210623..211615	+	993	WP_004673552.1	BMP family ABC transporter substrate-binding protein	-
Bra5_RS01005	211704..213215	+	1512	WP_037115036.1	ABC transporter ATP-binding protein	-
Bra5_RS01010	213223..214326	+	1104	WP_004673554.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-92)

Antitoxin

• Antitoxin ParD (1-79)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11043.52 Da
• Isoelectric Point: 6.0567

>T75645 WP_085741536.1 NZ_CP020896:209340-209630 [Rhizobium phaseoli Brasil 5]
MGFRLTEEAEEDIIGIAETGIRLFGSAHARHYHDELYAIFDLIAANPRMARERDEISPPMRIHPFKAHLVVYTIVEGDHV
LIVRIRHGHEDWSSQA

Nucleotide

• Length: 291 bp

>T75645 NZ_CP020896:209340-209630 [Rhizobium phaseoli Brasil 5]
ATGGGCTTTCGCCTTACGGAAGAAGCGGAGGAGGACATTATCGGCATAGCCGAAACCGGTATTCGCCTGTTTGGCTCAGC
GCACGCTCGGCACTACCATGATGAACTCTACGCCATTTTTGATCTGATTGCTGCCAATCCCCGTATGGCGCGCGAACGAG
ATGAAATTTCTCCTCCGATGCGCATCCATCCGTTCAAGGCCCATCTCGTCGTCTATACGATTGTGGAGGGCGATCATGTT
CTCATTGTCCGTATTCGCCACGGACACGAAGATTGGTCCAGTCAGGCTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9308.43 Da
• Isoelectric Point: 9.6900

>AT75645 WP_004673546.1 NZ_CP020896:209086-209337 [Rhizobium phaseoli Brasil 5]
MATMNVSLPDPMKDWVEAQTKTGRYANASDYVRDLIRKDQERNDKIAAMQRFVDEGLKSGVGSRSKDALFEAARARVESS
RGN

Nucleotide

• Length: 252 bp

>AT75645 NZ_CP020896:209086-209337 [Rhizobium phaseoli Brasil 5]
ATGGCGACCATGAACGTATCCCTGCCCGATCCCATGAAAGATTGGGTGGAAGCGCAAACCAAAACAGGCCGTTATGCCAA
TGCGAGCGATTATGTCCGCGACCTCATCAGGAAAGACCAGGAGCGAAACGATAAGATTGCCGCTATGCAGCGCTTTGTCG
ATGAAGGCTTGAAGAGCGGCGTCGGGTCGCGGTCGAAGGACGCACTGTTTGAGGCCGCGCGGGCGCGCGTGGAAAGCTCT
CGCGGCAATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0M3GP98