Detailed information of TA system TA075633

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 896283..896872
• Replicon: chromosome
• Accession: NZ_CP020889
• Organism: Xanthomonas citri pv. citri strain TX160197

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: B7L67_RS04140
• Protein ID: WP_015463824.1
• Coordinates: 896283..896615 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: B7L67_RS04145
• Protein ID: WP_011052949.1
• Coordinates: 896615..896872 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L67_RS26080	894763..894984	-	222	WP_011052944.1	hypothetical protein	-
B7L67_RS04130	895274..895810	-	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
B7L67_RS04135	895800..896012	-	213	WP_011052947.1	hypothetical protein	-
B7L67_RS25460	896009..896260	-	252	WP_125459571.1	hypothetical protein	-
B7L67_RS04140	896283..896615	-	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7L67_RS04145	896615..896872	-	258	WP_011052949.1	antitoxin	Antitoxin
B7L67_RS04150	897078..897680	+	603	WP_011052950.1	recombinase family protein	-
B7L67_RS04155	897664..897968	+	305	Protein_757	Tn3 family transposase	-
B7L67_RS04160	898135..901095	-	2961	WP_015472939.1	Tn3 family transposase	-
B7L67_RS04165	901140..901565	-	426	Protein_759	tyrosine-type recombinase/integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T75633 WP_015463824.1 NZ_CP020889:c896615-896283 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T75633 NZ_CP020889:c896615-896283 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT75633 WP_011052949.1 NZ_CP020889:c896872-896615 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT75633 NZ_CP020889:c896872-896615 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6