Detailed information of TA system TA075632

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 35664..36253
• Replicon: plasmid unnamed3
• Accession: NZ_CP020888
• Organism: Xanthomonas citri pv. citri strain TX160149

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: M4U123
• Locus tag: B7L66_RS25480
• Protein ID: WP_015463824.1
• Coordinates: 35921..36253 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: M4UHU6
• Locus tag: B7L66_RS25475
• Protein ID: WP_011052949.1
• Coordinates: 35664..35921 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L66_RS25460	31441..34401	+	2961	WP_059032461.1	Tn3 family transposase	-
B7L66_RS25465	34568..34872	-	305	Protein_35	Tn3 family transposase	-
B7L66_RS25470	34856..35458	-	603	WP_011052950.1	recombinase family protein	-
B7L66_RS25475	35664..35921	+	258	WP_011052949.1	antitoxin	Antitoxin
B7L66_RS25480	35921..36253	+	333	WP_015463824.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7L66_RS26200	36246..36527	+	282	WP_131826283.1	hypothetical protein	-
B7L66_RS25485	36524..36736	+	213	WP_011052947.1	hypothetical protein	-
B7L66_RS25490	36726..37262	+	537	WP_015463823.1	plasmid pRiA4b ORF-3 family protein	-
B7L66_RS26630	37552..37773	+	222	WP_011052944.1	hypothetical protein	-
B7L66_RS25505	38019..41195	+	3177	WP_088016387.1	TAL effector repeat-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48956	48956

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-108)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11707.46 Da
• Isoelectric Point: 9.1975

>T75632 WP_015463824.1 NZ_CP020888:35921-36253 [Xanthomonas citri pv. citri]
MDRGDVYLVSLDPTSGHEQQGTRPVLIVSPSAFNRLTKTPVVLPITSGGNFARTAGFTVSLMGAGTNTTGVVRCDQPRAL
DLASRRARKLETVPSPIMDEVLAKLSAILE

Nucleotide

• Length: 333 bp

>T75632 NZ_CP020888:35921-36253 [Xanthomonas citri pv. citri]
ATGGATCGCGGCGATGTCTATTTGGTTTCGCTCGATCCCACGTCCGGCCATGAACAACAGGGCACGCGCCCGGTGCTGAT
TGTGTCGCCAAGCGCGTTCAACCGCCTGACGAAAACACCGGTCGTGCTGCCGATCACCAGTGGGGGTAACTTCGCCCGCA
CGGCAGGCTTTACCGTGTCGCTCATGGGTGCGGGTACCAACACAACTGGCGTAGTTCGCTGCGATCAGCCCCGCGCTCTC
GATCTAGCCTCGCGGCGCGCGCGCAAGCTGGAAACCGTGCCGTCCCCGATCATGGATGAGGTATTAGCGAAGCTGAGCGC
GATCCTTGAGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9242.70 Da
• Isoelectric Point: 4.7164

>AT75632 WP_011052949.1 NZ_CP020888:35664-35921 [Xanthomonas citri pv. citri]
MHTTNLRKVGGSIMLAVPPAILDMLRLRAGATVGLAVDHGRLVIEPTLRPYYSLDELLAQCDASAELSTEDREWLDAKPV
GSELL

Nucleotide

• Length: 258 bp

>AT75632 NZ_CP020888:35664-35921 [Xanthomonas citri pv. citri]
ATGCATACGACCAATCTGCGTAAGGTCGGTGGCTCGATCATGTTGGCTGTCCCGCCCGCCATTCTGGATATGCTGCGTCT
GCGTGCCGGCGCTACCGTTGGCTTGGCCGTCGATCATGGTCGCCTGGTGATCGAACCCACGCTGCGTCCGTACTACAGCC
TGGATGAGCTGCTGGCCCAATGCGACGCATCCGCCGAACTCTCCACCGAAGATCGGGAATGGCTCGACGCCAAGCCTGTT
GGCAGCGAGCTGCTGTGA

Structures

Toxin

• AlphaFold DB: M4U123

Antitoxin

• AlphaFold DB: M4UHU6