Detailed information of TA system TA075630

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 7201..7742
• Replicon: plasmid unnamed3
• Accession: NZ_CP020888
• Organism: Xanthomonas citri pv. citri strain TX160149

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: B7L66_RS25305
• Protein ID: WP_015462614.1
• Coordinates: 7201..7527 (-)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H8FMA7
• Locus tag: B7L66_RS25310
• Protein ID: WP_003491150.1
• Coordinates: 7524..7742 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
B7L66_RS25295	2584..4158	+	1575	WP_039587421.1	type IV secretion system DNA-binding domain-containing protein	-
B7L66_RS25300	4172..7147	+	2976	WP_059032462.1	conjugative relaxase	-
B7L66_RS25305	7201..7527	-	327	WP_015462614.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
B7L66_RS25310	7524..7742	-	219	WP_003491150.1	antitoxin MazE family protein	Antitoxin
B7L66_RS25315	7879..8265	-	387	WP_003491149.1	hypothetical protein	-
B7L66_RS25320	8342..8761	-	420	WP_011052981.1	hypothetical protein	-
B7L66_RS26190	9047..9256	-	210	Protein_11	IS5/IS1182 family transposase	-
B7L66_RS25330	9483..9914	-	432	Protein_12	replication protein	-
B7L66_RS25335	9937..11313	-	1377	WP_011347842.1	IS5-like element ISXca5 family transposase	-
B7L66_RS25340	11432..12229	-	798	Protein_14	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..48956	48956
	-	inside	IScluster/Tn	-	-	9937..16028	6091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-103)

Antitoxin

• Antitoxin MazE-like (5-70)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11676.70 Da
• Isoelectric Point: 9.2550

>T75630 WP_015462614.1 NZ_CP020888:c7527-7201 [Xanthomonas citri pv. citri]
MMRGDFVTIAMQGDFGKPRPALVIQADQFDAHTTVTVLPVTSTLVAAPLLRITVHPSTDNGLQKPSQVMVDKAMTVKRDK
VGRAFGRVDADALVEIERCLAVFLGIAK

Nucleotide

• Length: 327 bp

>T75630 NZ_CP020888:c7527-7201 [Xanthomonas citri pv. citri]
ATGATGCGGGGTGACTTTGTGACCATCGCCATGCAAGGCGACTTCGGAAAACCAAGGCCCGCTCTGGTGATCCAGGCCGA
CCAGTTTGACGCACACACCACCGTGACGGTGCTGCCGGTGACAAGCACGCTCGTTGCTGCGCCGCTGCTGCGTATTACCG
TTCATCCAAGTACCGACAATGGTTTGCAGAAGCCGTCTCAGGTGATGGTGGACAAGGCCATGACCGTGAAGCGTGACAAG
GTAGGTCGAGCCTTTGGGCGCGTAGACGCTGATGCGTTGGTGGAAATTGAGCGCTGCCTGGCCGTGTTCTTGGGCATCGC
TAAGTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8418.54 Da
• Isoelectric Point: 5.6599

>AT75630 WP_003491150.1 NZ_CP020888:c7742-7524 [Xanthomonas citri pv. citri]
MAHVNSRVQKHRDALRMAGLRPVQIWVPDTRRPDFAEECRRQCRLAAQADMADTDMQRFMDEALADMDGWTE

Nucleotide

• Length: 219 bp

>AT75630 NZ_CP020888:c7742-7524 [Xanthomonas citri pv. citri]
ATGGCACACGTCAATTCACGAGTTCAAAAGCACCGCGACGCCCTGCGCATGGCAGGGCTGCGTCCGGTGCAAATTTGGGT
GCCGGACACACGGCGGCCTGACTTCGCCGAGGAATGCCGCCGTCAGTGTCGTCTTGCTGCACAAGCGGACATGGCGGATA
CCGACATGCAGCGCTTCATGGATGAGGCGCTAGCAGACATGGATGGCTGGACGGAATGA

Structures

Antitoxin

• AlphaFold DB: H8FMA7